Development of markers followed by construction of genetic linkage maps and quantitative trait locus (QTL) identification is a widely applied strategy towards marker assisted selection (MAS). In MPOB, researchers have reported that a restriction fragment length polymorphism (RFLP) marker, CB75A, generated from a OxG genetic linkage map showed significant correlation with QTL for iodine value (IV) and fatty acid composition (FAC) in oil palm. This indicated a potential use of the marker to assist breeding to develop genotypes with more liquid oil. The objective of this study is to validate the CB75A marker across various independent populations to gauge its utility for oil palm breeding. Palms were selected based on their palmitic acid composition, and classified as low, intermediate and high. Validation was carried out using RFLP technique on 18 Madagascar germplasm samples from Trial 0.240 at MPOB Kluang, 20 hybrids from Trial 0.236 at MPOB UKM and 60 backcross 1 (BC1) from Trial 0.336 at MPOB Kluang. Three bands were observed and assigned as A, B and C. The assay showed that the AA genotype is associated with low palmitic acid. In addition, significant segregation can only be observed in interspecific hybrid backcross samples. This inferred that although CB75A can predict FAC content to some extent, additional markers are required for a more precise prediction.