Journal of Oil Palm Research Vol. 31  March 2019 p.  159-164
DOI: https://doi.org/10.21894/jopr.2019.0002

ANTIOXIDANT PROPERTIES OF CRUDE AND COLD ETHANOL PRECIPITATED PROTEIN FROM PALM KERNEL CAKE (PKC) AS POTENTIAL COSMECEUTICAL AGENT

Author(s): CHOI, W C*; HON, W M**; MOHAMAD, M‡; KOK, A K D X‡; LAI, K S‡‡ and YAP, W S*

Palm kernel cake (PKC) is a by-product obtained from the production of edible oils using oil palm
(Elaeis guineensis). PKC is well-known for its high protein content, therefore, it was chosen as the target
of this research to study its antioxidant properties, which is an important criteria in cosmeceutical industry.
Our studies showed that, the extraction of crude protein at 80°C resulted in the highest total phenolic
content (TPC) and protein yield. It was further seen that precipitation using 80% cold ethanol following
protein extraction at 80°C gave the best protein yield of 56.6%. The antioxidative activity of this precipitated
protein was expressed as IC50 for 1-diphenyl 1-2-picrylhydrazyl (DPPH) radical-scavenging capacity
(517±0.016 mg ml-1), 2, 2′-azino-bisC3-ethylbenzothia zoline-6-sulphonic acid (ABTS) radical-scavenging
capacity (0.047±0.009 mg ml-1) and ferrous ion-chelating (FIC) ability (21±0.13 mg ml-1). Meanwhile, the
reducing power and TPC were 0.156±0.006 trolox equivalent antioxidant capacity (TEAC) mmol g-1 dry
weight (DW) and 25.10±0.58 gallic acid equivalent (GAE) μg g-1 DW respectively. The Pearson correlation
test further revealed a significantly moderate to strong positive relationships between antioxidant properties
with protein content and antioxidant properties with TPC. Taken together, both, the crude and precipitated
protein obtained from PKC showed substantial amount of antioxidative activities, which could be used as
sustainable source of antioxidant peptides in enhancing the quality of cosmeceutical products.

Keywords: , , , ,

Author Information
* Department of Biotechnology, Faculty of Applied Sciences, UCSI University, 1 Jalan Puncak Menara Gading, Taman Connaught, 56000 Kuala Lumpur, Malaysia.
E-mail: wsyap@ucsiuniversity.edu.my

** Vice-Chancellor’s Office, KDU University College, SS 22/41, Damansara Jaya,47400 Petaling Jaya, Selangor, Malaysia.

‡ University Council, UCSI University, 1 Jalan Puncak Menara Gading, 56000 Kuala Lumpur, Malaysia.

‡‡ Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.


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