Journal of Oil Palm Research Vol. 10 No. 2  1998 Dec p.  29-38

Biolistic-mediated DNA delivery and isolation of transgenic oil palm (Elaeis guineensis Jacq.) embryogenic callus cultures

Author(s): PARVEEZ, G K A*; CHRISTOU, P.**

Oil palm (Elaeis guineensis Jacq.) embryogenic calli were transformed with plasmids containing various com- binations of genes encoding β-glucuronidase, phosphinotricin acetyltransferase and hygromycin phosphotransferase, using an electric discharge particle bombardment device. Experiments to optimize the accelerating force for DNA delivery into embryogenic tissue of oil palm were carried out. Selection and culture conditions to allow recovery of transgenic tissue are reported. Stable integration of transgenes was confirmed by molecular analysis. Due to the long time required for regeneration of transgenic plants from oil palm embryogenic callus, this report is significant as it describes, for the first time, conditions leading to the recovery of transgenic embryogenic callus cultures carrying and expressing transformed marker genes.

Keywords: , , ,

Author Information
* Palm Oil Research Institute of Malaysia, P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.

** John Innes Centre, Norwich Research park, Colney, Norwich NR4 7UH, UK.

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