Category Archives: In Press

AN RFLP MARKER, CB75A, PREDICTS FOR PALMITIC ACID CONTENT ACROSS SELECTED OIL PALM GERMPLASM AND INTERSPECIFIC-HYBRIDS

Development of markers followed by construction of genetic linkage maps and quantitative trait locus (QTL) identification is a widely applied strategy towards marker assisted selection (MAS). In MPOB, researchers have reported that a restriction fragment length polymorphism (RFLP) marker, CB75A, generated from a OxG genetic linkage map showed significant correlation with QTL for iodine value (IV) and fatty acid composition (FAC) in oil palm. This indicated a potential use of the marker to assist breeding to develop genotypes with more liquid oil. The objective of this study is to validate the CB75A marker across various independent populations to gauge its utility for oil palm breeding. Palms were selected based on their palmitic acid composition, and classified as low, intermediate and high. Validation was carried out using RFLP technique on 18 Madagascar germplasm samples from Trial 0.240 at MPOB Kluang, 20 hybrids from Trial 0.236 at MPOB UKM and 60 backcross 1 (BC1) from Trial 0.336 at MPOB Kluang. Three bands were observed and assigned as A, B and C. The assay showed that the AA genotype is associated with low palmitic acid. In addition, significant segregation can only be observed in interspecific hybrid backcross samples. This inferred that although CB75A can predict FAC content to some extent, additional markers are required for a more precise prediction.

EFFECT OF FLOW RATE AND CONCENTRATION OF CARBAMIDE ON THE REDUCING NOx EMISSIONS IN PALM BIODIESEL FUELED RESEARCH ENGINE

This study examines the consequences of concentration and flow rate of carbamide (urea) to reduce NOx emissions from a diesel engine propelled with biodiesel. Taguchi-Grey relational-based multi-response optimisation method was employed for test-plan. Results revealed that carbamide flow rate and its concentration have a positive impact on NOx emissions reduction in unmodified diesel engine. The obtained results are also justified with results attained from ANOVA. It is also confirmed that the results obtained in the experimentations are in line with the calculated values obtained Taguchi technique. Hence, the usage of carbamide in tailpipe is an effective way in reducing NOx emissions from diesel engine.