Journal of Oil Palm Research Vol. 22  2010 December p.  856-868

Cloning and in silico analysis of promoters of highly expressed genes in oil palm embryogenic cultures

Author(s): OOI Siew Eng* ; FESHAH Ishak* ; MEILINA ONG Abdullah*

Promoters are the regulatory regions of DNA that control the expression of their genes. This study aimed at identifying the 5’ upstream regulatory regions of four embryogenically up-regulated genes that may elucidate clues to their regulation. Isolation and sequence analyses of the four putative promoters revealed similarities in the predicted motifs. The putative promoters of OPSC10 and EgHOX1 shared more motifs in common compared to the other two promoters, accounting for similarities in the two former expression profiles. The highly represented motifs in the putative promoters suggested regulation by light, phytohormones and Myb binding proteins. The upstream region of EgPER1 was the most unique of the four, with the possibility of being part of a dicistronic operon.

Keywords: , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

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