Journal of Oil Palm Research Vol. 21  2009 June p.  621-626

Concentration and isolation of individual vitamin E components in palm phytonutrients concentrate using high performance liqiud chromatography with flourescence detection

Author(s): CHANDRASEKARAM, Kumuthini * ; NG Mei Han ** ; CHOO Yuen May ** ; CHUAH Cheng Hock *

Palm phytonutrients concentrate is a rich source of vitamin E. Vitamin E content in palm phytonutrients concentrate was found to be 15 370 ppm. Components of vitamin E identified in the palm phytonutrients concentrate consists of α-tocopherol (α-T), γ-tocopherol (γ-T), α-tocotrienol (α-T3), γ-tocotrienol (γ-T3) and δ-tocotrienol (δ-T3). This article reports a study on the concentration of individual palm vitamin E components in crude palm oil (CPO), palm phytonutrients concentrate (PPC) and unsaponifiables of palm phytonutrients concentrate (unsap PPC). This article also reports a study on the concentration of bulk vitamin E through the application of open column chromatography and subsequent isolation of individual palm vitamin E components using High Performance Liquid Chromatography (HPLC) with fluorescence detection and a semi-preparative silica column. As the vitamin E components are concentrated in a stepwise manner by means of saponification, open column chromatography and semi-preparative HPLC, the concentration of individual components can be enriched by up to 94.6%.

Keywords: , , ,

Author Information
* Chemistry Department, Science Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia.

** Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

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