Journal of Oil Palm Research Vol. 26  2014 March p.  104-108
DOI:

Correlation of Microspore Nuclear Development with Male Inflorescence Morphology in Elaeis oleifera, Elaeis guineensis and the OxG Hybrid

Author(s): Latif, A M; Zaki*, N M; Nordiana*, H M N*; Marhalil, M*; Che Radziah, C M Z** and Madon, M*

Microspore nuclear stages of the Elaeis species and their OxG hybrid were exam­ined and cor­re­lated with the respec­tive male inflo­res­cence mor­phol­ogy. Iso­lated pollen mother cells (PMC) and microspores obtained from squashed male flower buds were stained with blue flu­o­res­cent 4’,6-diamidino-2-phenylindole (DAPI) dye and observed with the Zeiss Axio­plan epi­flu­o­res­cent micro­scope to deter­mine their nuclear stages. The male inflo­res­cence from both the Elaeis species and the (OxG) hybrid with a per­fectly closed spathe, hav­ing whitish inflo­res­cences and fri­able indi­vid­ual spikelets indi­cated that PMC activ­i­ties were ongo­ing within the anthers of male flower buds. In E. guineen­sis, the whitish, pale yel­low or light green spikelets with flex­i­ble tex­ture obtained from a per­fectly closed spathe appeared to be mor­pho­log­i­cal mark­ers for unin­u­cle­ate microspores. For E. oleifera inflo­res­cences, the colour varies from pale beige to umber brown with flex­i­ble spikelets, while the spathe var­ied from being per­fectly closed to slightly open. The observed mor­phol­ogy indi­cated the pres­ence of unin­u­cle­ate microspores in E. oleifera. For the OxG hybrid mor­pho­log­i­cal mark­ers resem­bled that of E. oleifera. By stain­ing a plethora of male flower buds from var­i­ous oil palm male inflo­res­cences, the meio­sis and mito­sis phases were also profiled.

Keywords: , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail: maria@mpob.gov.my

** Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.


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