Journal of Oil Palm Research Vol. 27  June 2015 p.  113-127
DOI:

DEVELOPMENT OF AN EFFECTIVE SSR-BASED FINGERPRINTING SYSTEM FOR COMMERCIAL PLANTING MATERIALS AND BREEDING APPLICATIONS IN OIL PALM

Author(s): WONG WEI CHEE*; TEO CHIN JIT**; WONG CHOO KIEN**; SEAN MAYES‡; RAJINDER SINGH‡‡ and SOH AIK CHIN‡

DNA fin­ger­prints pro­vide the basis for genetic diver­sity stud­ies and mol­e­c­u­lar breed­ing. How­ever, rou­tine and com­mer­cial scale DNA fin­ger­print­ing in oil palm (Elaeis guineen­sis Jacq.) remains chal­lenged by the need for a robust yet low cost method. In addi­tion, there is also a need for a pre­cise and reli­able marker set capa­ble of dis­tin­guish­ing oil palm geno­types within nar­row and mixed genetic pop­u­la­tion bases at the indi­vid­ual and pop­u­la­tion level. With the rapid evo­lu­tion of geno­typ­ing and sequenc­ing tech­nolo­gies, a wide range of geno­typ­ing tools have been devel­oped but are not yet com­mer­cially fea­si­ble for oil palm. Con­se­quently, we tested the fea­si­bil­ity of using sim­ple sequence repeat (SSR) mark­ers to geno­type oil palm breed­ing and com­mer­cial plant­ing mate­ri­als of restricted and mixed parent­age from Advanced Agrie­co­log­i­cal Research Sdn Bhd (AAR). The results obtained from screen­ing 33 palms using a selected set of 17 highly poly­mor­phic and infor­ma­tive SSR mark­ers obtained from an ini­tial set of 23 SSR, showed that clones, crosses and sibs within crosses could be sep­a­rated by their DNA fin­ger­prints. How­ever, as expected palms within clones could not be sep­a­rated. In another test exer­cise at the pop­u­la­tion scale, the four vari­able but related pop­u­la­tions: Deli; Dumpy Deli x Yangambi x AVROS; Dumpy Deli x AVROS; Dumpy Deli x AVROS x La Me; were dis­tinctly sep­a­rated using 11 SSR from the same pool of 23 poly­mor­phic SSR. There is good poten­tial for this set of 11 SSR mark­ers to be devel­oped for gen­eral applic­a­bil­ity across all E. guineen­sis oil palm genetic mate­ri­als and for use in the Malaysian Plant Vari­ety Pro­tec­tion Act. The set of SSR mark­ers also serve as the base set for the devel­op­ment of marker-assisted and genome-wide selec­tion at AAR.

Keywords: , ,

Author Information
* Biotechnology Section, Advanced Agriecological Research Sdn Bhd, AAR-UNMC Biotechnology Research Centre, Jalan Broga, 43500 Semenyih, Selangor, Malaysia. E-mail: wongwc@aarsb.com.my

** Plant Breeding Section, Advanced Agriecological Research Sdn Bhd, 86609 Paloh, Johor, Malaysia.

‡ Crops for the Future Research Centre, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia.

‡‡ Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.


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