Journal of Oil Palm Research Vol. Special Issue  2008 April p.  23-34
DOI:

Effect of agitation and aeration on yield optimization of oil palm suspension culture

Author(s): DAE-SUNG Choi * ; ANDRADE, Maria Helena Cano * ; WILLIS, Laura B * ; CHANGWON Cho * ; SCHOENHEIT, Joerg ** ; BOCCAZZI, Paolo * ; SAMBANTHAMURTHI, Ravigadevi + ; SINSKEY, Anthony J * ; CHOKYUN Rha *

Oil palm is an oil producing tropical plant. While it is the highest yielding oil producing plant, it takes three years to yield. Therefore there is an urgent need for controlled production for oil palm suspension cells in a bioreactor to support the clonal propagation of elite planting materials. The propagation of the cells is affected by various culture conditions. In this study, we examined the effect of two operating parameters – agitation rate (80 to 335 rpm) and aeration (10% to 80% dissolved oxygen) – on the proliferation of suspension cells. The sugar consumption, production of biomass, amino acids and organic acids were monitored. At 43% dissolved oxygen, two of the agitation rates (120 rpm and 225 rpm) resulted in more than 200% increase in biomass compared to the initial inoculum. This study indicates that the bioreactor operating parameters between 120-300 rpm and 20%-80% dissolved oxygen are acceptable for the culture of oil palm cells.

Keywords: , , , ,

Author Information
* Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
E-mail: ckrha@mit.edu

** Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

+ Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 1-Oil Palm Tissue Culture


Cited By

Call For Papers

Article In Press

Search for:


Most cited articles


SIGN UP To JOPR MAILING LIST

Subscribe with us to get the latest information on Palm Oil Research from MPOB today!