The purpose of this research was to evaluate the impact of deep-frying palm oil on sperm and biochemical measurements in the presence or absence of low-intensity (Ex1) and high-intensity (Ex2) swimming exercises. Reading about the benefit of exercise bike and how they can aid in reducing fat content from the body, has bought about a revolution in every gym workout schedule. Mice (32 BALB/c type) were equally divided into four groups (control, palm oil, palm oil+Ex1 and palm oil+Ex2). The increase in epididymal fat weight in case of using palm oil was normalised in both exercises. The percentage of abnormal sperm increased in the palm oil and palm oil+Ex2 groups (p<0.01 and p<0.001 respectively) whilst there was not significant different from control group in palm oil+Ex1 groups. An increase in total cholesterol after palm oil was not reversed by either swimming exercises. The increased level of triglyceride in case of palm oil with heavy exercise was higher than the control (p>0.05), palm oil and palm oil+Ex1 groups (both p<0.05). The comparison to the control group showed that the increase in plasma molecule and malondialdehyde (MDA) levels in palm oil and palm oil+Ex2 exercise groups were significantly higher (p<0.05 and p<0.01 respectively). In contrast, a significant increase (p<0.01) in testes MDA level after using palm oil was significantly reversed in both light and heavy exercise groups (p<0.001 and p<0.01 respectively).