Two Trichoderma harzianum strains (FA 1132 and FA1166) were tested as biocontrol agents for basal stem rot in oil palm seedlings artificially infected with the causal pathogen, Ganoderma boninense. The treatment was carried out by applying a Trichoderma–infused surface mulch and periodic applications of a conidial soil drech made from spore suspensions of the respective Trichoderma strains. A disease severity index (DSI) ranging from 0 to 100 was used to assess the disease severity. A single strain application of T. harzianum, FA 1132 gave the best disease suppression with the lowest DSI of 28.35 compared to the infected, non-treated control plants that gave the highest DSI of 86.67. However, FA 1166 as a single application was ineffective, so was the mixture of the two strains. The biological control property of Trichoderma was shown to be strain-specific and not species-specific. In addition, it was found that applying the mixed inocula significantly decresed the performance of FA 1132, the choice strain.
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
** Biology Department, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
+ Plant Protection Department, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
1. Krauss, U., et al. "Mycoparasitism by Clonostachys Byssicola and Clonostachys Rosea on Trichoderma Spp. from Cocoa (Theobroma Cacao) and Implication for the Design of Mixed Biocontrol Agents." Biological Control 67(3) (2013): 317-327.
2. Naher, L., et al. "Cloning of Transcripts Encoding Chitinases from Elaeis Guineensis Jacq. and their Expression Profiles in Response to Fungal Infections." Physiological and Molecular Plant Pathology 76(2) (2011): 96-103.
3. Naher, L., et al. "Biocontrol Agent Trichoderma Harzianum Strain FA 1132 as an Enhancer of Oil Palm Growth." Pertanika Journal of Tropical Agricultural Science 35(1) (2012): 173-82.
4. Shariffah-Muzaimah, S. A., et al. "Isolation of Actinomycetes from Rhizosphere of Oil Palm (Elaeis Guineensis Jacq.) for Antagonism Against Ganoderma Boninense." Journal of Oil Palm Research 27(1) (2015): 19-29.
5. Sundram, S. "First Report: Isolation of Endophytic Trichoderma from Oil Palm (Elaeis Guineensis Jacq.) and their in Vitro Antagonistic Assessment on Ganoderma Boninense." Journal of Oil Palm Research 25(DEC) (2013): 368-372.
6. Sundram, S., et al. "Symbiotic Interaction of Endophytic Bacteria with Arbuscular Mycorrhizal Fungi and its Antagonistic Effect on Ganoderma Boninense." Journal of Microbiology 49(4) (2011): 551-557.
7. Yeoh, K. -A, et al. "Sequence Analysis and Gene Expression of Putative Oil Palm Chitinase and Chitinase-Like Proteins in Response to Colonization of Ganoderma Boninense and Trichoderma Harzianum." Molecular biology reports 40(1) (2013): 147-158.
8. Anuar, E. N., R. Nulit, and A. S. Idris. "Growth Promoting Effects of Endophytic Fungus Phlebia GanoEF3 on Oil Palm (Elaeis Guineensis) Seedlings." International Journal of Agriculture and Biology 17(1) (2015): 135-41.
9. Ho, C. -L, et al. "De Novo Transcriptome Analyses of Host-Fungal Interactions in Oil Palm (Elaeis Guineensis Jacq.)." BMC Genomics 17(1) (2016)
10. Marzuki, N. F., et al. "Evaluation on the Cultural Characteristics and Antagonistic Activities of Cladobotryum Semicirculare Against Ganoderma Boninense in Vitro."Journal of Oil Palm Research 27(4) (2015): 326-338.
11. Rakib, M. R. M., et al. "Aggressiveness of Ganoderma Boninense and G. Zonatum Isolated from Upper - and Basal Stem Rot of Oil Palm (Elaeis Guineensis) in Malaysia." Journal of Oil Palm Research 27(3) (2015): 229-240.
12. Sundram, S., et al. "Application of Arbuscular Mycorrhizal Fungi with Pseudomonas Aeruginosa UPMP3 Reduces the Development of Ganoderma Basal Stem Rot Disease in Oil Palm Seedlings." Mycorrhiza 25.5 (2015): 387-397.
13. Tan, Y. -C, M. -Y Wong, and C. -L Ho. "Expression Profiles of Defence Related cDNAs in Oil Palm (Elaeis Guineensis Jacq.) Inoculated with Mycorrhizae and Trichoderma Harzianum Rifai T32." Plant Physiology and Biochemistry 96 (2015): 296-300.
14. Ting, A. S. Y., and J. Y. Chai. "Chitinase and β-1,3-Glucanase Activities of Trichoderma Harzianum in Response Towards Pathogenic and Non-Pathogenic Isolates: Early Indications of Compatibility in Consortium." Biocatalysis and Agricultural Biotechnology 4(1) (2015): 109-113.
15. Angel, L. P. L., et al. "An in Vitro Study of the Antifungal Activity of Trichoderma Virens 7b and a Profile of its Non-Polar Antifungal Components Released Against Ganoderma Boninense." Journal of Microbiology 54(11) (2016): 732-744.
16. Goh, Y. K., et al. "Mycoparasitic Scytalidium Parasiticum as a Potential Biocontrol Agent Against Ganoderma Boninense Basal Stem Rot in Oil Palm*." Biocontrol Science and Technology 26(10) (2016): 1352-1365.
Last updated: 21 November 2016
Subscribe with us to get the latest information on Palm Oil Research from MPOB today!