Journal of Oil Palm Research Vol. 20 June  2008 June p.  470-483
DOI:

Efficacy of single and mixed treatments of Trichoderma harzianum as biocontrol agents of Ganoderma basal stem rot in oil palm

Author(s): SHAMALA Sundram * ; FARIDAH Abdullah ** ; ZAINAL ABIDIN Mior Ahmad + ; UMI KALSOM Yusuf **

Two Trichoderma harzianum strains (FA 1132 and FA1166) were tested as biocontrol agents for basal stem rot in oil palm seedlings artificially infected with the causal pathogen, Ganoderma boninense. The treatment was carried out by applying a Trichoderma-infused surface mulch and periodic applications of a conidial soil drech made from spore suspensions of the respective Trichoderma strains. A disease severity index (DSI) ranging from 0 to 100 was used to assess the disease severity. A single strain application of T. harzianum, FA 1132 gave the best disease suppression with the lowest DSI of 28.35 compared to the infected, non-treated control plants that gave the highest DSI of 86.67. However, FA 1166 as a single application was ineffective, so was the mixture of the two strains. The biological control property of Trichoderma was shown to be strain-specific and not species-specific. In addition, it was found that applying the mixed inocula significantly decresed the performance of FA 1132, the choice strain.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
E-mail: shamala@mpob.gov.my

** Biology Department, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

+ Plant Protection Department, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.


Cited By

(21)
1) Ho, C.-L., Tan, Y.-C., Yeoh, K.-A., Lee, W.-K., Ghazali, A.-K., Yee, W.-Y., Hoh, C.-C. Transcriptional response of oil palm (Elaeis guineensis Jacq.) inoculated simultaneously with both Ganoderma boninense and Trichoderma harzianum (2018) Plant Gene, 13, pp. 56-63.
2) Page, D.E., Glen, M., Puspitasari, D., Rimbawanto, A., Ratkowsky, D., Mohammed, C. Sexuality and mating types of Ganoderma philippii, Ganoderma mastoporum and Ganoderma australe, three basidiomycete fungi with contrasting ecological roles in south-east Asian pulpwood plantations (2018) Australasian Plant Pathology, 47 (1), pp. 83-94.
3) Shariffah-Muzaimah, S.A., Idris, A.S., Madihah, A.Z., Dzolkhifli, O., Kamaruzzaman, S., Maizatul-Suriza, M. Characterization of Streptomyces spp. isolated from the rhizosphere of oil palm and evaluation of their ability to suppress basal stem rot disease in oil palm seedlings when applied as powder formulations in a glasshouse trial (2018) World Journal of Microbiology and Biotechnology, 34 (1), art. no. 15, .
4) Dislich, C., Keyel, A.C., Salecker, J., Kisel, Y., Meyer, K.M., Auliya, M., Barnes, A.D., Corre, M.D., Darras, K., Faust, H., Hess, B., Klasen, S., Knohl, A., Kreft, H., Meijide, A., Nurdiansyah, F., Otten, F., Pe'er, G., Steinebach, S., Tarigan, S., Tölle, M.H., Tscharntke, T., Wiegand, K. A review of the ecosystem functions in oil palm plantations, using forests as a reference system (2017) Biological Reviews, 92 (3), pp. 1539-1569.
5) Nurazah, Z., Idris, A.S., Kushairi, A., Din, A.M., Abrizah, O., Ramli, U.S. Metabolomics unravel differences between Cameroon Dura and deli Dura oil palm (Elaeis guineensis Jacq.) genetic backgrounds against Basal stem rot (2017) Journal of Oil Palm Research, 29 (2), pp. 227-241.
6) Angel, L.P.L., Yusof, M.T., Ismail, I.S., Ping, B.T.Y., Mohamed Azni, I.N.A., Kamarudin, N.H., Sundram, S. An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense (2016) Journal of Microbiology, 54 (11), pp. 732-744.
7) Goh, Y.K., Marzuki, N.F., Goh, T.K., Tan, S.Y., Goh, Y.K., Goh, K.J. Mycoparasitic Scytalidium parasiticum as a potential biocontrol agent against Ganoderma boninense basal stem rot in oil palm* (2016) Biocontrol Science and Technology, 26 (10), pp. 1352-1365.
8) Ho, C.-L., Tan, Y.-C., Yeoh, K.-A., Ghazali, A.-K., Yee, W.-Y., Hoh, C.-C. De novo transcriptome analyses of host-fungal interactions in oil palm (Elaeis guineensis Jacq.) (2016) BMC Genomics, 17 (1), art. no. 66, .
9) Sundram, S., Meon, S., Seman, I.A., Othman, R. Application of arbuscular mycorrhizal fungi with Pseudomonas aeruginosa UPMP3 reduces the development of Ganoderma basal stem rot disease in oil palm seedlings (2015) Mycorrhiza, 25 (5), pp. 387-397.
10) Marzuki, N.F., Goh, Y.K., Tung, H.J., Goh, Y.K., Goh, K.J. Evaluation on the cultural characteristics and antagonistic activities of cladobotryum semicirculare against ganoderma boninense in vitro (2015) Journal of Oil Palm Research, 27 (4), pp. 326-338.
11) Tan, Y.-C., Wong, M.-Y., Ho, C.-L. Expression profiles of defence related cDNAs in oil palm (Elaeis guineensis Jacq.) inoculated with mycorrhizae and Trichoderma harzianum Rifai T32 (2015) Plant Physiology and Biochemistry, 96, pp. 296-300.
12) Rakib, M.R.M., Bong, C.F.J., Khairulmazmi, A., Idris, A.S. Aggressiveness of Ganoderma boninense and G. Zonatum isolated from upper - And Basal stem rot of oil palm (Elaeis guineensis) in Malaysia (2015) Journal of Oil Palm Research, 27 (3), pp. 229-240.
13) Shariffah-Muzaimah, S.A., Idris, A.S., Madihah, A.Z., Dzolkhifli, O., Kamaruzzaman, S., Cheong, P.C.H. Isolation of actinomycetes from rhizosphere of oil palm (Elaeis guineensis Jacq.) for antagonism against Ganoderma boninense (2015) Journal of Oil Palm Research, 27 (1), pp. 19-29.
14) Anuar, E.N., Nulit, R., Idris, A.S. Growth promoting effects of endophytic fungus Phlebia GanoEF3 on oil palm (Elaeis guineensis) seedlings (2015) International Journal of Agriculture and Biology, 17 (1), pp. 135-141.
15) Ting, A.S.Y., Chai, J.Y. Chitinase and β-1,3-glucanase activities of Trichoderma harzianum in response towards pathogenic and non-pathogenic isolates: Early indications of compatibility in consortium (2015) Biocatalysis and Agricultural Biotechnology, 4 (1), pp. 109-113.
16) Krauss, U., ten Hoopen, M., Rees, R., Stirrup, T., Argyle, T., George, A., Arroyo, C., Corrales, E., Casanoves, F. Mycoparasitism by Clonostachys byssicola and Clonostachys rosea on Trichoderma spp. from cocoa (Theobroma cacao) and implication for the design of mixed biocontrol agents (2013) Biological Control, 67 (3), pp. 317-327.
17) Sundram, S. First report: Isolation of endophytic trichoderma from oil palm (Elaeis guineensis Jacq.) and their in vitro antagonistic assessment on Ganoderma boninense (2013) Journal of Oil Palm Research, 25 (DEC), pp. 368-372.
18) Yeoh, K.-A., Othman, A., Meon, S., Abdullah, F., Ho, C.-L. Sequence analysis and gene expression of putative oil palm chitinase and chitinase-like proteins in response to colonization of Ganoderma boninense and Trichoderma harzianum (2013) Molecular Biology Reports, 40 (1), pp. 147-158.
19) Naher, L., Tan, S.G., Yusuf, U.K., Ho, C.-L., Abdullah, F. Biocontrol agent trichoderma harzianum strain FA 1132 as an enhancer of oil palm growth (2012) Pertanika Journal of Tropical Agricultural Science, 35 (1), pp. 173-182.
20) Sundram, S., Meon, S., Seman, I.A., Othman, R. Symbiotic interaction of endophytic bacteria with arbuscular mycorrhizal fungi and its antagonistic effect on Ganoderma boninense (2011) Journal of Microbiology, 49 (4), pp. 551-557.
21) Naher, L., Ho, C.-L., Tan, S.G., Yusuf, U.K., Abdullah, F. Cloning of transcripts encoding chitinases from Elaeis guineensis Jacq. and their expression profiles in response to fungal infections (2011) Physiological and Molecular Plant Pathology, 76 (2), pp. 96-103.

Source: Scopus
Last updated: 30 July 2018

Call For Papers

Article In Press

Search for:


Most cited articles


SIGN UP To JOPR MAILING LIST

Subscribe with us to get the latest information on Palm Oil Research from MPOB today!