Journal of Oil Palm Research Vol. 27 (2) June 2015, p. 135-143

ENZYME PROFILE OF THE OIL PALM (Elaeis guineensis Jacq.) PEST Coelaenomenodera lameensis Berti AND Mariau (Coleoptera: chryomelidae, hispinae) ACCORDING TO THE DIFFERENT DEVELOPMENT STAGES



Coelaenomenodera lameensis Berti and Mariau, (Coleoptera, Chrysomelidae) is the most important oil palm (Elaeis guineensis Jacq.) leaf miner pest in Ivory Coast,West Africa. The leaf miner activity of C. lameensis was higher in instars 1-3 (L1-L3) larvae. Analysis of the enzymatic profiles indicated that instars 1 and 2 larvae are the most powerful ones in polysaccharides degradation activity, higher gain being obtained with xylan (28 μg of glucose equivalent released per minute (U) per milligramme of protein) and laminarin (18 U mg-1). The most important heterosidasic activities were detected in L3 larvae and to a lesser extent in L1-2. The β-glucoside was hydrolysed at an activity rate higher than 400 U mg-1 by L3 larvae. The β-xylosidasic and N-acetyl-glucosaminidasic activities were respectively 39.13 and 35.81 U mg-1 for L1-2 and 34.9 and 36.39 U mg-1 for L3 larvae. The data suggested that the majority of carbohydrate digestion occurs in the L1-2, and L3 instars. Enzymatic profiles of L4 larvae and adults were characterised by low activities. Finally, the feeding behaviour (foraging activity) and the enzymatic activities were all linked, underlying the digestive capability of each developmental stage of C. lameensis. The main digestive enzymes of this pest are identified, the search for inhibitors of these enzymes may be considered.


* Entomology and Soil Fauna Unity, UFR Biosciences, Félix Houphouet-Boigny University, Abidjan (Côte d’Ivoire) 22 BP: 1611 Abidjan 22, Ivory Coast.

** BIOEMCO-IBIOS, University of Paris Est Créteil, 61 Avenue du Général de Gaulle, 94010 Créteil Cedex, France. E-mail: