Journal of Oil Palm Research Vol. 27  December 2015 p.  326-338
DOI:

EVALUATION ON THE CULTURAL CHARACTERISTICS AND ANTAGONISTIC ACTIVITIES OF Cladobotryum semicirculare AGAINST Ganoderma boninense in vitro

Author(s): NURUL FADHILAH MARZUKI*; YIT KHENG GOH*; HUN JIAT TUNG**; YOU KENG GOH* and KAH JOO GOH*

The objec­tives of this pre­lim­i­nary study were to iden­tify the Cladobotryum-like iso­late and assess the antag­o­nis­tic activ­ity of this fun­gus against G. boni­nense and other fun­gal pathogens. Based on both mor­pho­log­i­cal and mol­e­c­u­lar approaches, the unknown fun­gus was later described as Cladobotryum semi­cir­cu­lare. Under dual-culture assays, Cladobotryum semi­cir­cu­lare was observed to sup­press radial mycelial growth of all the tested fungi with G. lucidum and G. boni­nense G37’s growth being
inhib­ited the most, 74.8% and 74.7%, respec­tively. Clonos­tachys sp. inhib­ited the least (12.7%). Through poi­son agar test, fil­trates from C. semi­cir­cu­lare at 50% con­cen­tra­tion were observed to sup­press the mycelial growth of G. boni­nense G14 (35% inhi­bi­tion) and G. lucidum (25% sup­pres­sion), and with 100% fil­trate con­cen­tra­tion both Gan­o­derma species were show­ing more than 49% inhi­bi­tion. Growth
of all tested Gan­o­derma iso­lates and C. semi­cir­cu­lare, was faster under aer­o­bic (2.2 to 3.8 mm per day for Gan­o­derma iso­lates and 4.3 mm for Cladobotryum) com­pared to anaer­o­bic (1.6 to 2.2 mm per day for Gan­o­derma iso­lates and 1.4 mm per day for Cladobotryum) con­di­tion. In vitro myco­par­a­sitism test con­firmed that C. semi­cir­cu­lare reduced the re-isolation of G. lucidum (33.3%) and G. boni­nense (66.7%) mycelia. This study also pro­vided the first report of G. boni­nense as the host for C. semicirculare.

Keywords: , , , ,

Author Information
* Advanced Agriecological Research Sdn Bhd, 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, 47810 Petaling Jaya, Selangor, Malaysia. E-mail: gohykheng@aarsb.com.my

** Applied Agricultural Resources Sdn Bhd (AAR), University of Nottingham Malaysia Campus (UNMC), Biotechnology Research Centre, Jalan Broga 43500 Semenyih, Selangor, Malaysia.


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