Journal of Oil Palm Research Vol. 20 June  2008 June p.  495-507

Factors affecting green fluorescence protein (GFP) gene expression in oil palm after microprojectile bombardment

Author(s): AHMAD PARVEEZ Ghulam Kadir* ; NA'IMATULAPIDAH Abdul Majid*

Expression of green fluorescence protein (GFP) gene can be visualized under ultraviolet or blue light without any sunstrate or co-factor addition. It has been used to monitor transient and stable transgene expression in manuy plant varieties. The effectiveness of gfp gene as a selectable marker for oil palm transformation was evaluated through transient expression of gfp gene in bombarded oil palm embryogenic calli and immature embyros. Different types (version) of gfp gene which are driven by different constitutive promoters were used to transform oil palm target tissues. Some of the gfp genes used were targeted to specific organelle: namely plastid, endoplasmic reticulum and mittochondria. Transient expression of the gfp genes could be detected in oil palm tissues as early as 16 hr after bombardment. It was observed that the number of gfp expressing cells and duration of the gfp gene expression differs from one construct to another. The differences in the gfp constructs performance in oil palm tissues were evaluated based on the following factors: version of the gfp genes, promoter used to drive the gfp gene, backbone vector and the size of the whole plasmid. The CaMV35S promoter was found to be the most effective promoter for driving gfp gene in oil palm tissues followed by HBT and maize ubiquitin promoter. The sGFPS65T was the most effective version of gfp gene for oil palm tissues followed by sGFP and mGFP5. It was also demonstrated that the pUC18 backbone vectors was the most effective vector backbone in expressing the gfp gene in oil palm. Finally, it was observed that the smaller the gfp vector, the higher the number of gfp expressing cells obtained. Possible reasons for these observations were elaborated and discussed.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

Cited By

1. Chaparro-Pulido, C. A., et al. "Development of an Efficient Transient Transformation Protocol for Avocado (Persea Americana Mill.) Embryogenic Callus." In Vitro Cellular and Developmental Biology - Plant, Vol. 50, No. 2, 2014:292-298, doi:10.1007/s11627-013-9564-2.
2. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science, Vol. 6, No. September, 2015, doi:10.3389/fpls.2015.00727.
3. Majid, N. A., and G. K. A. Parveez. "Regeneration of Transgenic Oil Palm Carrying GFP Gene used as a Visual Selectable Marker." Journal of Oil Palm Research, Vol. 28, No. 4, 2016:415-430.
4. Nurfahisza, A. R., et al. "Comparison of the Effectiveness of Basta, Bialaphos and Glufosinate Ammonium for Selecting Transformed Oil Palm Tissues." Journal of Oil Palm Research, Vol. 28, No. 3, 2016:247-255.
5. Parveez, G. K. A., et al. "Tissue Culture and Genetic Engineering of Oil Palm." Palm Oil: Production, Processing, Characterization, and Uses. , 2012, doi:10.1016/B978-0-9818936-9-3.50007-1.
6. Parveez, G. K. A., et al. "Biotechnology of Oil Palm: Strategies Towards Manipulation of Lipid Content and Composition." Plant Cell Reports, Vol. 34, No. 4, 2015:533-543, doi:10.1007/s00299-014-1722-4.
7. Wan Nur Syuhada, W. S., O. A. Rasid, and G. K. A. Parveez. "Evaluation on the Effects of Culture Medium on Regeneration of Oil Palm Plantlets from Immature Embryos (IE)." Journal of Oil Palm Research, Vol. 28, No. 2, 2016:234-239.

Source: Scopus
Last Updated: 31 May 2017

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