Journal of Oil Palm Research Vol.   p.  
DOI: https://doi.org/10.21894/jopr.2018.0044

GENETIC DIVERSITY AND INBREEDING LEVEL IN DELI Dura AND AVROS ADVANCED BREEDING MATERIALS IN OIL PALM (Elaeis guineensis Jacq.) USING MICROSATELLITE MARKERS

Author(s): TAY CHEE CHUN*; CHUA KIA LING*; LEE CHONG HEE*; CHEAH SUAN CHOO**; JAKIM BARIN‡; AU WAI FONG‡; MOHD PUAD ABDULLAH‡‡ and TAN SOON GUAN‡‡

Oil palm has a narrow genetic diversity due to intensive selection in breeding. Loss of diversity in breeding materials can lead to many consequences, despite uniformity is critical to breeders. The objectives of the study are to evaluate the genetic diversity in Deli and AVROS populations, and to determine the levels of inbreeding using CIRAD’s Simple Sequence Repeat (SSR) markers. The parental populations evaluated were 186 palms from Deli and 188 palms from AVROS progenies sourced from two agencies. Genotyping using 32 SSR markers produced 230 alleles among the 17 oil palm progenies. The number of alleles scored ranged between 4 to 11. The average number of alleles per locus was 7.1875. The expected heterozygosity (He) and observed heterozygosity (Ho) were 0.7063 and 0.5270, respectively. The dendogram revealed that the 17 populations were clustered into three main clusters namely Deli dura, AVROS and Ekona populations. The two different generations of AVROS resulted in Genting AgTech Sdn Bhd (GAT) AVROS had inbreeding coefficient of 0.0336 compared to Department of Agriculture (DOA) Sabah AVROS which was -0.0810. The diversity revealed via SSR showed that there are still present of variability in some of the progenies. The inbreeding level can serve as guidance during the inbred lines development.

Keywords: , ,

Author Information
* Genting AgTech Sdn Bhd, Lot L3-I-1, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
E-mail:cheechun224@gmail.com

** ACGT Sdn Bhd, Lot L3-I-1, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.

‡ Department of Agriculture Sabah, Level 5, Wisma Pertanian Sabah, Jalan Tasik, Off Jalan Maktab Gaya, 88632 Kota Kinabalu, Sabah, Malaysia.

‡‡ Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.


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