Journal of Oil Palm Research Vol. 18  2006 June p.  189-197

Identification of lutein in crude palm oil and evaluation of carotenoids at various ripening stages of the oil palm fruit

Author(s): BONNIE TAY Yen Ping * ; GWENDOLINE, E.C.L. **

The xanthophylls in crude palm oil (CPO) were identified as lutein, α-carotene 5,6-epoxide, α-carotene 5,8-epoxide, and β-carotene 5,6-epoxide and/or β-carotene 5’,6’-epoxide based on their electronic absorption maxima from HPLC-PDA. Lutein, a dihydroxy carotenoid, was isolated from CPO extract and its structure confirmed by co-elution with an authentic standard using HPLC-photodiode array (PDA) and mass spectra. At 7 DAA (days after anthesis), lutein was predominant with only trace amounts of α- and β-carotenes. At 21, 84 and 126 DAA, the levels of α- and β-carotenes increased although lutein still predominated. However, when the palm fruit ripens at 140 DAA, the level of lutein decreased dramatically to a minor carotene while α- and β-carotenes predominated with other minor carotenes, i.e. phytoene, lycopene, β-zeacarotene, γ-carotene, ζ-carotene, formed.

Keywords: , , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

** Universiti Putra Malaysia, Department of Chemistry, Faculty of Science and Environment, 43400 UPM Serdang, Selangor, Malaysia.

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