Journal of Oil Palm Research Vol. 25  2013 December p.  368-372
DOI:

First Report: Isolation of Endophytic Trichoderma from Oil Palm (Elaeis guineensis Jacq.) and Their in vitro Antagonistic Assessment on Ganoderma boninense

Author(s): SHAMALA SUNDRAM*

The iso­la­tion of endo­phytic Tri­cho­derma from dif­fer­ent tis­sues viz. leaf, rachis, stem and root of oil palm (Elaeis guineen­sis Jacq.) was inves­ti­gated in this study. A total of six palms were sam­pled from two plan­ta­tion plots and it was found that Tri­cho­derma iso­lates were specif­i­cally colonis­ing the stems and roots of oil palm and none were found in the leaves and rachis. Tri­cho­derma selec­tive media E (TME) was used for iso­la­tion. Iden­ti­fi­ca­tion of the iso­lates was fur­ther estab­lished using slide cul­tures. A total of 40 iso­lates were obtained with 10 from stem and 30 from root sam­ples of oil palm. All iso­lates were sub­jected to dual cul­ture assay; a pre­lim­i­nary assay to screen myco­paratisms poten­tial through antag­o­nis­tic activ­ity against Gan­o­derma boni­nense. Sub­se­quently, seven poten­tial Tri­cho­derma iso­lates were tested for their antibio­sis prop­er­ties through poi­son food agar assay. This is the first report of endo­phytic Tri­cho­derma iso­lated from oil palm with bio­con­trol poten­tial against G. boni­nense.

Keywords: , , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
E-mail: shamala@mpob.gov.my


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