Journal of Oil Palm Research Vol. 25  2013 December p.  368-372

First Report: Isolation of Endophytic Trichoderma from Oil Palm (Elaeis guineensis Jacq.) and Their in vitro Antagonistic Assessment on Ganoderma boninense


The isolation of endophytic Trichoderma from different tissues viz. leaf, rachis, stem and root of oil palm (Elaeis guineensis Jacq.) was investigated in this study. A total of six palms were sampled from two plantation plots and it was found that Trichoderma isolates were specifically colonising the stems and roots of oil palm and none were found in the leaves and rachis. Trichoderma selective media E (TME) was used for isolation. Identification of the isolates was further established using slide cultures. A total of 40 isolates were obtained with 10 from stem and 30 from root samples of oil palm. All isolates were subjected to dual culture assay; a preliminary assay to screen mycoparatisms potential through antagonistic activity against Ganoderma boninense. Subsequently, seven potential Trichoderma isolates were tested for their antibiosis properties through poison food agar assay. This is the first report of endophytic Trichoderma isolated from oil palm with biocontrol potential against G. boninense.

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Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.

Cited By

1. Angel, L. P. L., et al. "An in Vitro Study of the Antifungal Activity of Trichoderma Virens 7b and a Profile of its Non-Polar Antifungal Components Released Against Ganoderma Boninense." Journal of Microbiology, Vol. 54, No. 11, 2016:732-744, doi:10.1007/s12275-016-6304-4.
2. Chow, Y. Y., S. Rahman, and A. S. Y. Ting. "Understanding Colonization and Proliferation Potential of Endophytes and Pathogen in Planta Via Plating, Polymerase Chain Reaction and Ergosterol Assay." Journal of Advanced Research, Vol. 8, No. 1, 2017:13-21, doi:10.1016/j.jare.2016.10.008.
3. Goh, Y. K., et al. "Mycoparasitic Scytalidium Parasiticum as a Potential Biocontrol Agent Against Ganoderma Boninense Basal Stem Rot in Oil Palm." Biocontrol Science and Technology, Vol. 26, No. 10, 2016:1352-1365, doi:10.1080/09583157.2016.1202192.
4. Marzuki, N. F., et al. "Evaluation on the Cultural Characteristics and Antagonistic Activities of Cladobotryum Semicirculare Against Ganoderma Boninense in Vitro." Journal of Oil Palm Research, Vol. 27, No. 4, 2015:326-338.
5. Naidu, Y., et al. "In Vitro Antagonistic Interactions between Endophytic Basidiomycetes of Oil Palm (Elaeis Guineensis) and Ganoderma Boninense." Journal of Phytopathology, Vol. 164, No. 10, 2016:779-790, doi:10.1111/jph.12498.
6. Parthiban, K., et al. "GIS Mapping of Basal Stem Rot Disease in Relation to Soil Series among Oil Palm Smallholders." American Journal of Agricultural and Biological Science, Vol. 11, No. 1, 2016:2-12, doi:10.3844/ajabssp.2016.2.12.

Source: Scopus
Last Updated: 31 May 2017

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