Journal of Oil Palm Research Vol. 25  2013 December p.  368-372

First Report: Isolation of Endophytic Trichoderma from Oil Palm (Elaeis guineensis Jacq.) and Their in vitro Antagonistic Assessment on Ganoderma boninense


The iso­la­tion of endo­phytic Tri­cho­derma from dif­fer­ent tis­sues viz. leaf, rachis, stem and root of oil palm (Elaeis guineen­sis Jacq.) was inves­ti­gated in this study. A total of six palms were sam­pled from two plan­ta­tion plots and it was found that Tri­cho­derma iso­lates were specif­i­cally colonis­ing the stems and roots of oil palm and none were found in the leaves and rachis. Tri­cho­derma selec­tive media E (TME) was used for iso­la­tion. Iden­ti­fi­ca­tion of the iso­lates was fur­ther estab­lished using slide cul­tures. A total of 40 iso­lates were obtained with 10 from stem and 30 from root sam­ples of oil palm. All iso­lates were sub­jected to dual cul­ture assay; a pre­lim­i­nary assay to screen myco­paratisms poten­tial through antag­o­nis­tic activ­ity against Gan­o­derma boni­nense. Sub­se­quently, seven poten­tial Tri­cho­derma iso­lates were tested for their antibio­sis prop­er­ties through poi­son food agar assay. This is the first report of endo­phytic Tri­cho­derma iso­lated from oil palm with bio­con­trol poten­tial against G. boni­nense.

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Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.

Cited By

1. Angel, L. P. L., et al. "An in Vitro Study of the Antifungal Activity of Trichoderma Virens 7b and a Profile of its Non-Polar Antifungal Components Released Against Ganoderma Boninense." Journal of Microbiology, Vol. 54, No. 11, 2016:732-744, doi:10.1007/s12275-016-6304-4.
2. Chow, Y. Y., S. Rahman, and A. S. Y. Ting. "Understanding Colonization and Proliferation Potential of Endophytes and Pathogen in Planta Via Plating, Polymerase Chain Reaction and Ergosterol Assay." Journal of Advanced Research, Vol. 8, No. 1, 2017:13-21, doi:10.1016/j.jare.2016.10.008.
3. Goh, Y. K., et al. "Mycoparasitic Scytalidium Parasiticum as a Potential Biocontrol Agent Against Ganoderma Boninense Basal Stem Rot in Oil Palm." Biocontrol Science and Technology, Vol. 26, No. 10, 2016:1352-1365, doi:10.1080/09583157.2016.1202192.
4. Marzuki, N. F., et al. "Evaluation on the Cultural Characteristics and Antagonistic Activities of Cladobotryum Semicirculare Against Ganoderma Boninense in Vitro." Journal of Oil Palm Research, Vol. 27, No. 4, 2015:326-338.
5. Naidu, Y., et al. "In Vitro Antagonistic Interactions between Endophytic Basidiomycetes of Oil Palm (Elaeis Guineensis) and Ganoderma Boninense." Journal of Phytopathology, Vol. 164, No. 10, 2016:779-790, doi:10.1111/jph.12498.
6. Parthiban, K., et al. "GIS Mapping of Basal Stem Rot Disease in Relation to Soil Series among Oil Palm Smallholders." American Journal of Agricultural and Biological Science, Vol. 11, No. 1, 2016:2-12, doi:10.3844/ajabssp.2016.2.12.

Source: Scopus
Last Updated: 31 May 2017

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