RESEARCH ARTICLE

Journal of Oil Palm Research Vol. 3 No. 2 1991 Dec, p. 349-354

ISOZYME ANALYSIS OF PLANTLETS FROM SEVEN CLONES OF OIL PALM (ELAEIS GUINEENSIS JACQ.)

CHEE Seng Ooi*; KHENG Choo Wooi+; AM Pai Chong*

ABSTRACT

Starch gel electrophoresis of shoot extracts from plantlets of seven clones of oil palm (Elaeis guineensis Jacq.) was carried out and 12 enzyme systems were examined. Polymorphisms were detected for glutamate-oxaloacetate-transaminase(GOT),isocitrate dehydrogenase(IDH), leucine amino-peptidase (LAP), phosphohexose isomerase (PHI) and shikimate dehydrogenase (SHDH). Within clones, no differences could be detected. Using GOT, IDH, and SHDH as genetic markers, five of the clones could be differentiated, with the remaining two clones showing no isozyme differences between them.

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* Departments of Genetic Cellular Biology,
University of Malaya,
59100 Kuala Lumpur, Malaysia.

+ Tissue Culture Laboratory,
Ebor Research, Sime Darby Plantations,
P O Box 147, 41910 Klang,
Selangor, Malaysia.