Large scale production of octyl-9,10-dihydroxystearate was performed in a 5-litre batch, stirred tank reactor with a multi-bladed propeller. The effects of reaction temperature, reaction time, enzyme dosage and agitation speed were studied. The optimal conditions for the ester synthesis were reaction temperature at 50ºC, reaction time of 3 hr, catalyst concentration of 10% (w/w) and agitation speed of 300 rpm. The percentage conversion was about 90% with respect to the amount of dihydroxystearic acid (DHSA) used. The operational stability of the catalyst was also evaluated