The aim of the ecotoxicity test was to investigate the effect of Lepcon-1, Bafog-1 (S) and Ecobac-1 (EC) against the freshwater fish, Tilapia nilotica. The ecotoxicity test assessed the toxicity of these products by exposing T. nilotica to various concentrations of the sample. Media preparation and toxicity test was done according to the Organisation for Economic Cooperation and Development (OECD) 203 Fish, Acute Toxicity Test (OECD) Guidelines for Testing of Chemicals (1992). The results showed that the exposure of Lepcon-1, Bafog-1 (S) and Ecobac-1 (EC), each product at 320 mg litre-1, did not cause any mortality to T. nilotica after 96 hr of exposure. Therefore, these three products are considered non-toxic to T. nilotica.