Journal of Oil Palm Research Vol. 26 (1) March 2014, p. 96-103 A Rahman Nurfahisza*; Md Aman Rafiqah*; Mat Yunus Abdul Masani*; Ayub Nor Hanin*; Omar Abdul Rasid*; Ghulam Kadir Ahmad Parveez* and Ismanizan Ismail**
In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP-thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.KEYWORDS:
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru
Bangi, 43000 Kajang, Selangor, Malaysia.
** School of Biosciences and Biotechnology,
Universiti Kebangsaan Malaysia,
43650 UKM Bangi,Selangor, Malaysia.