Journal of Oil Palm Research Vol. 26  2014 March p.  96-103
DOI:

Molecular Analysis of Transgenic Oil Palm to Detect the Presence of Transgenes

Author(s): A Rahman Nurfahisza*; Md Aman Rafiqah*; Mat Yunus Abdul Masani*; Ayub Nor Hanin*; Omar Abdul Rasid*; Ghulam Kadir Ahmad Parveez* and Ismanizan Ismail**

In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP-thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail: parveez@mpob.gov.my

** School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43650 UKM Bangi,Selangor, Malaysia.


Cited By

(6)
1. Hanin, A. N., M. Y. A. Masani, and G. K. A. Parveez. "Evaluation of Oil Palm Leaf-Specific Promoter (LSP1) Activity for Expressing Phb Genes in Arabidopsis Thaliana." Journal of Oil Palm Research, Vol. 28, No. 1, 2016:1-9.
2. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science, Vol. 6, No. September, 2015, doi:10.3389/fpls.2015.00727.
3. Majid, N. A., and G. K. A. Parveez. "Regeneration of Transgenic Oil Palm Carrying GFP Gene used as a Visual Selectable Marker." Journal of Oil Palm Research, Vol. 28, No. 4, 2016:415-430.
4. Nurfahisza, A. R., et al. "Comparison of the Effectiveness of Basta, Bialaphos and Glufosinate Ammonium for Selecting Transformed Oil Palm Tissues." Journal of Oil Palm Research, Vol. 28, No. 3, 2016:247-255.
5. Parveez, G. K. A., et al. "Production of Polyhydroxybutyrate in Oil Palm (Elaeis Guineensis Jacq.) Mediated by Microprojectile Bombardment of PHB Biosynthesis Genes into Embryogenic Calli." Frontiers in Plant Science, Vol. 6, No. AUG, 2015, doi:10.3389/fpls.2015.00598.
6. Parveez, G. K. A., et al. "Biotechnology of Oil Palm: Strategies Towards Manipulation of Lipid Content and Composition." Plant Cell Reports, Vol. 34, No. 4, 2015:533-543, doi:10.1007/s00299-014-1722-4.


Source: Scopus
Last Updated: 31 May 2017

Call For Papers

Article In Press

Search for:


Most cited articles


SIGN UP To JOPR MAILING LIST

Subscribe with us to get the latest information on Palm Oil Research from MPOB today!