Journal of Oil Palm Research Vol. 18  2006 Dec p.  253-259

Palm carotenoids profile as a quality control tool for palm carotene producers: introducing an imporvised methods by HPLC-photodiode array and A C30 column

Author(s): BONNIE TAY Yen Ping*

This study establishes a more detailed HPLC profile of palm carotenoidsthan that possible from reverse phase C18 column analysis that detects 11 types of carotenes but can only partially resolve the cis/trans geometrical isomers. Carotenoid extracts of palm pressed fibre, crude palm oil (CPO) and commercial red palm olein (CRPo) were analysed by HPLC-photodiote array detection using a reverse phase C30 column and the palm carotenoids elution sequence was found to be lutein, neuroporene (trans), neurosporene (cis), α-zeacarotene (cis), α-zeacarotene (trans), α-zeacarotene (cis), phytoene, phytofluene, β-zeacarotene, 13 and 13′ cis α-carotene, 13 cis β-carotene, trans α-carotene, 9 cis α-carotene, trans β-carotene, δ-carotene a (cis), δ-carotene b (cis), δ-carotene (trans), γ-carotene a (cis), γ-carotene(trans), γ-carotene b (cis), lyopene (cis) and lycopene (trans). However, ξ-carotene was not detected using this new method The run time for complete analysis was about 200 min.

Keywords: ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

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