Journal of Oil Palm Research Vol. 3 No. 2 1991 Dec, p. 363-368 KULKARNI, A S*; KHOTPAL, R R*; BHAKARE, H A*
The phospholipid and glycolipid compositions of oil from two varieties of the oil palm, Elaeis guineensis, namely dura and pisifera, are reported. The oil was extracted from mesocarp with chloroform-methanol (2:1, v/v) and the total phospholipids and total glycolipids were isolated from it by silicic acid column chronmtography using chloroform, methanol and acetone as the eluting solvents. The individual components of total phospholipids were identified after thin layer chromatography by comparison with authentic standards and using specific spray reagents. The mqior components were found to be phosphatidyl choline PC, 34% to 35%, phosphatidyl ethanolamine PE, 22% to 26%, phosphatidyl inositol PI, 21% to 25%, cardiolipin CL, 7% to 8%, phosphatidyl glycerol PG, 5% to 7%, and unidentified substances 4% to 6 per cent. The predominant fatty acids in all these components were palmitic, stearic, oleic and linoleic acids.
Thin layer chromatography revealed that the major components of total glycolipids were monoglycosyl diglyceride (MGDG), 22% to 25% diglycosyl diglyceride (DGDG), 42% to 45%, steryl glycoside (SG)13% to 14% and acylated steryl glycoside (ASG) 14% to 15%, with, unidentified substances making up to 2% to 9 percent. The predominant fatty acids of the glycolipids were palmitic, stearic, oleic, linoleic and linolenic acids. The SG and ASG fractions showed the presence of β-sitosterol, stigmasterol, campesterol and brassicasterol; β-sitosterol being the major component. For SG the ratio of sugar:sterol was 1:1 and for ASG the ratio of sugar:sterol:fatty acids was 1:1:1.
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* Department of Oil Technology,
Laxminarayan Institute of Technology,