Journal of Oil Palm Research Vol. 17 Dec  2005 Dec p.  110-123

Polyploidt induction of oil palm through colchicine and oryzalin treatments

Author(s): MARIA Madon * ; CLYDE, M.M. ** ; HASHIM, H ** ; MOHD YUSUF, Y ** ; MAT, H ** ; SARATHA, S **

Germinated oil palm seeds were treated with two antimitotic chemicals, colchicine and oryzalin, to induce polyploidy. The colchicine concentrations used varied from 2.5 to 10.0 mM, and the oryzalin concentrations from 15 µM to 120µM. The incubation period vary inversely with the chemical concentration from 48 to 6 hr. Colchicine treatments produced nine tetraploids, two triploids and a number of mixoploids. The oryzalin treatments produced four triploids and many mixoploids of 2n+3n and 3n+4n. However, one-way ANOVA showed that none of the treatments were able to induce polyploidy efficiently. Flow cytometry was found to be the most efficient method for detecting induced changes in the genome size or polyploidy level compared to stomata and chromosome counts. One-way ANOVA showed no significant difference in the stomata density between polyploids and controls (2n) while for plant morphology, polyploids seemed to be significantly shorter than their respective controls.

Keywords: , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

** Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43650 UKM Bangi, Selangor, Malaysia.

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