For the first time a phototrophic bacterium, Rhodovulum sulfidophilum grown in palm oil mill effluent (POME-PB) was successfully used as food for rotifers (Brachionus rotundiformis) and Artemia nauplii which were then fed to the larvae of the marble goby, Oxyeleotris marmorata. The cultivation of POME-PB is cheap and easy where it can be easily produced in sealed, plastic ziplock bags exposed to light, giving a harvestable biomass of 2.58 ± 0.34 g litre^-1 after 60 hr post-inoculation. Rotifers fed the biomass of settled bacteria (sPOME-PB) had comparable mean density (221 ± 9 rotifers ml^-1) as rotifers fed with microalgae Nannochloropsis sp. (212 ± 27 rotifers ml^-1). However, unsettled bacterial culture (uPOME-PB) fed to rotifers resulted in higher rotifer densities of up to 898 ± 489 rotifers ml^-1. By feeding the marble goby larvae with rotifers and Artemia nauplii cultured in uPOME-PB, a mean survival of 81.9 ± 3.0% was obtained for 30-day post-hatch fish larvae. This survival was much higher than fish larvae given rotifers and Artemia nauplii fed sPOME-PB (42.5 ± 9.0%; 71.4 ± 20.5%) and Nannochloropsis sp. (46.8 ± 2.9%). POME-PB thus has the potential to be an aquaculture feed for the future.