A biosurfactant has been successfully synthesized from fructose and palm fatty acid distillate (PFAD) with Lipozyme IM as biocatalyst. Analysis of its physical and chemical properties showed that this surfactant had a melting point of 49°C-52.3°C and a hydrophile-lipophile balance (HLP) of 16+. This HLB value is in the range suitable for use in cosmetics, detergents and foods. This surfactant, which was a fructose ester, also reduced the surface tension of water from 74 dynes cm^-1 to 38.3 dynes cm^-1.