Journal of Oil Palm Research Vol. 28  December 2016 p.  415-430
DOI: https://doi.org/10.21894/jopr.2016.2804.03

REGENERATION OF TRANSGENIC OIL PALM CARRYING GFP GENE USED AS A VISUAL SELECTABLE MARKER

Author(s): NA’IMATULAPIDAH ABDUL MAJID* and GHULAM KADIR AHMAD PARVEEZ*

Green flu­o­res­cent pro­tein (GFP) has been widely used as a visual selec­table marker for plant trans­for­ma­tion. Trans­formed cells could be eas­ily screened directly under ultraviolet/blue light with­out the need for any solu­tion or sub­strate. In this study, oil palm embryo­genic calli were bom­barded with a trans­for­ma­tion vec­tor, p35SCaMV-sgfpS65T, car­ry­ing a mod­i­fied ver­sion of gfp gene dri­ven by the 35S pro­moter. Upon bom­bard­ment, the expres­sion of gfp in embryo­genic calli was mon­i­tored visu­ally while the regen­er­a­tion of the embryo­genic calli was on-going. Regen­er­ated plantlets on medium with­out selec­tion agent demon­strated that only some parts of the plants showed GFP expres­sion. When gfp gene was co-bombarded with plas­mid pAHC25 car­ry­ing Basta resis­tant gene (bar), and the trans­formed embryo­genic calli were regen­er­ated on medium con­tain­ing her­bi­cide Basta, regen­er­ated plantlets obtained also showed GFP expres­sion in some parts of the plantlets instead of in the whole plant. Mol­e­c­u­lar analy­sis revealed the pres­ence of gfp trans­gene in the trans­genic plants. The pos­si­ble rea­sons for the fail­ure to obtain the whole plant express­ing gfp gene will be discussed.

Keywords: , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail: naima@mpob.gov.my


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