Acetylated glucose fatty esters (AGFE) were synthesized from palm and palm kernel fatty acid methyl esters (FAME) through a one-stage solvent-free interesterfication reaction with glucose pentaacetate (GPA). Two main products obtained and identified were mono-and di-substituted AGFEE. The hydrophile-lipophile balance (HLB), ternary phase diagram, cytotoxicity and antimicrobial bioassays were studied.