Journal of Oil Palm Research Vol. 7 No. 2, December 1995, p. 107-117 TARMIZI, A. H.*; MARZIAH, M.**
Low temperatures (15ºC-20ºC) were observed to inhibit the growth and multiplication of oil palm polyembryogenic (PE) cultures. These conditions enable PE cultures to be kept for at least six months without subculturing. The method can, therefore be exploited for minimal growth storage of in vitro cultures. Proline, which is known to be a universal stress indicator, was found to accumulate in the cultures under low temperature stress and the increase was about two-fold during 30 days of exposure. In the presence of 0.5 M sucrose, low temperature storage of cultures could be extended even to nine months.KEYWORDS:
* Palm Oil Research Institute of Malaysia,
P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.
** Department of Biochemistry & Microbiology,
Faculty of Science and Environmental Studies,
Universiti Pertanian Malaysia,
43400 UPM Serdang, Selangor, Malaysia.