A certain amount of crude palm oil (CPO) still remained in the palm pressed fibre (PPF) after going through the conventional milling processes. The use of aqueous enzymatic oil extraction (AEOE) was to recover the oil, hence, leading to the increase in oil extraction rate. With regards to the oil quality, the extracted PPF oil was analysed and compared to CPO using the MPOB Test Methods. From the results, the extracted oil contained high concentration of carotenoid (2750-2800 ppm) and vitamin E (930-950 ppm). Ironically, the quality was slightly lower than CPO, of which the values of deterioration of bleachability index (DOBI), free fatty acid (FFA) content and peroxide value (PV) were 1.12±0.12, 8.51±0.64% and 4.73±0.16 meq kg-1, respectively. With the exception of linolenic acid (C18:3), the fatty acid composition (FAC) of extracted oil was similar to CPO. Despite the oil quality, the use of an enzymatic route has the potential for the extraction of phytonutrient-rich oil from PPF.