Journal of Oil Palm Research Vol. Special Issue  2008 July p.  77-86
DOI:

Transformation of PHB and PHBV genes driven by maize ubiquitin promoter into oil palm for the production of biodegradable plastics

Author(s): AHMAD PARVEEZ Ghulam Kadir* ; BAHARIAH Bohari* ; NOR HANIN Ayub* ; ABDUL MASANI Mat Yunus* ; OMAR Abdul Rasid* ; AHMAD TARMIZI Hashim* ; ZAMZURI Ishak* ; MOHAMAD ARIF Abd Manaf* ; AHMAD KUSHAIRI Din* ; YORK, Gregory**; YEONG Bae Jo**; SINSKEY, Anthony J.**

Three bacterial genes coding for the enzymes, 3-ketothiolase (bktB), acetoacetyl-CoA reductase (phaB) and PHB synthase (phaC), required for the synthesis of PHB from acetyl-CoA in bacteria were transformed into oil palm embryogenic calli. For the production of copolymer polyhydroxybutyrate–co-valerate (PHBV), the threonine dehydratase (tdcB) gene from Escherichia coli was also transformed into oil palm embryogenic calli for producing propionyl-CoA, the substrate for hydroxyvalerate. These genes were under the control of the maize ubiquitin promoter. Currently, many transformed embryogenic lines resistant to the herbicide Basta have already been produced. These transformed calli were later regenerated to produce a few hundred plantlets which are now growing in a biosafety screenhouse. Molecular analyses have demonstrated stable integration of the transgenes in their genome.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
E-mail: parveez@mpob.gov.my

** Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering


Cited By

(13)
1. Ahmad Parveez, G. K., et al. "Improving Value of Oil Palm using Genetic Engineering." Biocatalysis and Biomolecular Engineering., 2010. 67-81.
2. Ariffin, N., et al. "Constructions of Expression Vectors of Polyhydroxybutyrate-Co-Hydroxyvalerate (PHBV) and Transient Expression of Transgenes in Immature Oil Palm Embryos." Plasmid 66(3) (2011): 136-43.
3. Chanprateep, S. "Current Trends in Biodegradable Polyhydroxyalkanoates." Journal of Bioscience and Bioengineering 110(6) (2010): 621-632.
4. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science 6(September) (2015)
5. Masani, M. Y. A., et al. "Efficient Transformation of Oil Palm Protoplasts by PEG-Mediated Transfection and DNA Microinjection." PLoS ONE 9(5) (2014)
6. Mat Yunus, A. M., G. K. Ahmad Parveez, and C. -L Ho. "Review: Transgenic Plants Producing Polyhydroxyalkanoates." Asia-Pacific Journal of Molecular Biology and Biotechnology 16(1) (2008): 1-10.
7. Ngando-Ebongue, G. F., et al. "Oil Palm." Technological Innovations in Major World Oil Crops. 1 Vol. , 2012. 165-200.
8. Parveez, G. K. A., et al. "Production of Polyhydroxybutyrate in Oil Palm (Elaeis Guineensis Jacq.) Mediated by Microprojectile Bombardment of PHB Biosynthesis Genes into Embryogenic Calli." Frontiers in Plant Science 6(AUG) (2015)
9. Parveez, G. K. A., et al. "Tissue Culture and Genetic Engineering of Oil Palm." Palm Oil: Production, Processing, Characterization, and Uses., 2012. 87-135.
10. Parveez, G. K. A., et al. "Biotechnology of Oil Palm: Strategies Towards Manipulation of Lipid Content and Composition." Plant Cell Reports 34.4 (2015): 533-543.
11. Snell, K. D., and O. P. Peoples. "PHA Bioplastic: A Value-Added Coproduct for Biomass Biorefineries." Biofuels, Bioproducts and Biorefining 3(4) (2009): 456-467.
12. Somleva, M. N., O. P. Peoples, and K. D. Snell. "PHA Bioplastics, Biochemicals, and Energy from Crops." Plant Biotechnology Journal 11(2) (2013): 233-52.
13. Corley, R. H. V., and P. B. Tinker. "The Oil Palm: Fifth Edition." The Oil Palm: Fifth Edition., 2015. 1-655.


Source: Scopus
Last checked: 22 May 2017

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