Journal of Oil Palm Research Vol. Special Issue  2008 July p.  77-86
Transformation of PHB and PHBV genes driven by maize ubiquitin promoter into oil palm for the production of biodegradable plastics

Author(s): AHMAD PARVEEZ Ghulam Kadir* ; BAHARIAH Bohari* ; NOR HANIN Ayub* ; ABDUL MASANI Mat Yunus* ; OMAR Abdul Rasid* ; AHMAD TARMIZI Hashim* ; ZAMZURI Ishak* ; MOHAMAD ARIF Abd Manaf* ; AHMAD KUSHAIRI Din* ; YORK, Gregory**; YEONG Bae Jo**; SINSKEY, Anthony J.**

Three bac­te­r­ial genes cod­ing for the enzymes, 3-ketothiolase (bktB), acetoacetyl-CoA reduc­tase (phaB) and PHB syn­thase (phaC), required for the syn­the­sis of PHB from acetyl-CoA in bac­te­ria were trans­formed into oil palm embryo­genic calli. For the pro­duc­tion of copoly­mer polyhydroxybutyrate–co-valerate (PHBV), the thre­o­nine dehy­dratase (tdcB) gene from Escherichia coli was also trans­formed into oil palm embryo­genic calli for pro­duc­ing propionyl-CoA, the sub­strate for hydrox­y­valer­ate. These genes were under the con­trol of the maize ubiq­ui­tin pro­moter. Cur­rently, many trans­formed embryo­genic lines resis­tant to the her­bi­cide Basta have already been pro­duced. These trans­formed calli were later regen­er­ated to pro­duce a few hun­dred plantlets which are now grow­ing in a biosafety screen­house. Mol­e­c­u­lar analy­ses have demon­strated sta­ble inte­gra­tion of the trans­genes in their genome.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
E-mail: parveez@mpob.gov.my

** Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering


Cited By

1. Ahmad Parveez, G. K., et al. "Improving Value of Oil Palm using Genetic Engineering." Biocatalysis and Biomolecular Engineering., 2010. 67-81.
2. Ariffin, N., et al. "Constructions of Expression Vectors of Polyhydroxybutyrate-Co-Hydroxyvalerate (PHBV) and Transient Expression of Transgenes in Immature Oil Palm Embryos." Plasmid 66(3) (2011): 136-43.
3. Chanprateep, S. "Current Trends in Biodegradable Polyhydroxyalkanoates." Journal of Bioscience and Bioengineering 110(6) (2010): 621-632.
4. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science 6(September) (2015)
5. Masani, M. Y. A., et al. "Efficient Transformation of Oil Palm Protoplasts by PEG-Mediated Transfection and DNA Microinjection." PLoS ONE 9(5) (2014)
6. Mat Yunus, A. M., G. K. Ahmad Parveez, and C. -L Ho. "Review: Transgenic Plants Producing Polyhydroxyalkanoates." Asia-Pacific Journal of Molecular Biology and Biotechnology 16(1) (2008): 1-10.
7. Ngando-Ebongue, G. F., et al. "Oil Palm." Technological Innovations in Major World Oil Crops. 1 Vol. , 2012. 165-200.
8. Parveez, G. K. A., et al. "Production of Polyhydroxybutyrate in Oil Palm (Elaeis Guineensis Jacq.) Mediated by Microprojectile Bombardment of PHB Biosynthesis Genes into Embryogenic Calli." Frontiers in Plant Science 6(AUG) (2015)
9. Parveez, G. K. A., et al. "Tissue Culture and Genetic Engineering of Oil Palm." Palm Oil: Production, Processing, Characterization, and Uses., 2012. 87-135.
10. Parveez, G. K. A., et al. "Biotechnology of Oil Palm: Strategies Towards Manipulation of Lipid Content and Composition." Plant Cell Reports 34.4 (2015): 533-543.
11. Snell, K. D., and O. P. Peoples. "PHA Bioplastic: A Value-Added Coproduct for Biomass Biorefineries." Biofuels, Bioproducts and Biorefining 3(4) (2009): 456-467.
12. Somleva, M. N., O. P. Peoples, and K. D. Snell. "PHA Bioplastics, Biochemicals, and Energy from Crops." Plant Biotechnology Journal 11(2) (2013): 233-52.
13. Corley, R. H. V., and P. B. Tinker. "The Oil Palm: Fifth Edition." The Oil Palm: Fifth Edition., 2015. 1-655.


Source: Scopus
Last updated: 22 November 2016

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