https://jopr.mpob.gov.my Mon, 30 Jun 2025 03:53:35 +0000 en-US hourly 1 https://wordpress.org/?v=7.0 https://jopr.mpob.gov.my/endogenous-hormones-from-the-young-leaves-of-oil-palm-ielaeis-guineensisi-jacq-a-response-to-iin-vitroi-callus-and-embryoid-induction/ Fri, 20 Jan 2023 01:50:14 +0000 https://jopr.mpob.gov.my/?p=12784 Oil palm is a plant that produces vegetable oil. Quality oil palm seedlings with superior characteristics can be produced clonally using tissue culture techniques. The selection of explant sources is very important, and one of the explant sources that can be used is the young oil palm leaves. The ability of callogenesis and embryogenesis remains low, and this is thought to be related to endogenous hormones. This study is aimed to identify the types of endogenous hormones in explants of young clonal oil palm leaves and their relationship to callus and embryoid induction. The leaf midribs of six young oil palms were selected as explant sources. Explants were extracted and analysed for endogenous hormone content using high-performance liquid chromatography. The results of the analysis obtained various types of endogenous hormones in four oil palm clones. Zeatin hormones, ABA and IBA were found in two clones. Nine hormones were found in the other two clones, namely zeatin, kinetin, gibberellic acid, IAA, BAP, ABA, IBA, 2,4-dichlorophenoxyacetic acid, and naphthalene acetic acid with various concentrations. Young oil palm leaves with a more complete content of endogenous hormones have the potential to produce embryogenic callus in greater numbers and can produce embryoids to ensure that they can support the production of plantlets using tissue culture technology.

]]> https://jopr.mpob.gov.my/determining-the-optimal-concentration-of-mannose-as-an-effective-selection-agent-for-transformed-oil-palm-cells-using-the-phosphomannose-isomerase-pmi-gene-as-a-positive-selectable-marker/ Sat, 13 Nov 2021 08:03:01 +0000 https://jopr.mpob.gov.my/V2/?p=9027 The elimination of antibiotic or herbicide resistance gene usage in genetically modified plants is being encouraged due to public concern. In response to this, alternative selection systems for the recovery of transgenic oil palm were developed using positive selectable markers. To establish a selection system that utilises the phosphomannose isomerase (pmi) gene for oil palm transformation, we first determined the optimal mannose concentration for selecting the transformed cells. Non-transformed embryogenic calli were cultured on media containing various combinations and concentrations of mannose and a usable source of carbon, i.e. sucrose, ranging in content from 0 to 30 g litre^-1. Sucrose is often used as a carbon source in plant tissue culture media. The embryogenic calli were subcultured onto similar fresh media every four weeks, and growth was recorded monthly up to five months. From the 10 combinations of mannose and sucrose evaluated, mannose:sucrose at 30:0 g litre^-1 was shown to be the most effective for selection because at this concentration the least plant growth was demonstrated for non-transformed embryogenic calli. We will thereafter use this particular concentration of mannose to select for oil palm embryogenic calli transformed with the pmi gene using biolistic bombardment.

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