https://jopr.mpob.gov.my Wed, 17 Nov 2021 07:44:41 +0000 en-US hourly 1 https://wordpress.org/?v=7.0 https://jopr.mpob.gov.my/effects-of-metsulfuron-methyl-on-als-activity-and-its-metabolism-in-immature-oil-palm/ Sat, 13 Nov 2021 08:13:49 +0000 https://jopr.mpob.gov.my/V2/?p=9104 The effects of metsulfuron-methyl on injury and acetolactate synthase (ALS) activity were measured in immature oil plam seedlings under greenhouse conditions. Metsulfuron-methyl sprayed directly onto seedlings caused a whole range of injuries to the 18-month-old palm seedlings, but its residue in soil did not affect seedling growth. Chlorosis appeared three weeks after treatment (WAT). The percentage of injury score was found to increase proportionally with herbicide dosage. Metsulfuron-methyl inhibited ALS enzyme in the seedlings. Metsulfuron-methyl at 41nM could inhibit 50% activity (I₅₀) of ALS enzyme. Metsulfuron-methyl compound was metabolized to seven metabolities, of which two could not be identified

]]> https://jopr.mpob.gov.my/identification-of-oil-palm-elaeis-guineensis-spear-leaf-metabolites-using-mass-spectrometry-and-neutral-loss-analysis/ Sat, 13 Nov 2021 07:58:11 +0000 https://jopr.mpob.gov.my/V2/?p=8987 Plant metabolites characterisation is an arduous challenge due to their structural diversity as a result of complicated biosynthetic pathways. These metabolites are not only important for metabolic events description but are also harnessed as valuable nutraceuticals. The detection and description of important plant tissue metabolome such as oil palm spear leaf can be carried out using a broad-range, non-targeted analytical tool such as mass spectrometry (MS). Identification of oil palm spear leaf constituents such as carboxyl group- and sugar- containing metabolites was facilitated by tandem mass spectrometry (MS/ MS) with neutral loss information of 44 and 162 amu. A total of 13 metabolites ranging from carboxylic acids, catechins, phenolic acid glycosides and a stilbenoid were characterised in this manner and the results presented here demonstrated the usefulness of MS in characterising metabolites in a complex sample such as oil palm spear leaf tissue.

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