Aluminium (Al) toxicity is one of the problems experienced by crops cultivated on acid soil, inhibiting root growth and nutrient absorption capability. This study evaluated the effects of different concentrations of Al-toxicity (0 μM-control; 100, 200 and 300 μM) on the physiological growth, chlorophyll content and selected nutrient uptake in roots and shoots of different varieties of oil palm seedlings, specifically the Elite Deli Dura x BM119 AVROS Pisifera, Elite Deli Dura x Elite AVROS Pisifera, Ulu Remis Deli Dura x Dumpy AVROS and Ulu Remis Deli Dura x Ulu Remis Tenera varieties. Results showed that Al-toxicity has no significant effect on the height of shoots for all oil palm seedling varieties. The Ulu Remis Deli Dura x Ulu Remis Tenera seedlings appeared to be more tolerant, showing no significant effect on bole diameter, chlorophyll content and biomass of both shoot and roots upon Al-toxicity treatment. For selected nutrient uptake, all tested oil palm seedling varieties showed significant effects upon Al-toxicity treatment primarily from the 100 μM Al-toxicity application. The findings from this study suggested that the Elite Deli Dura x BM119 AVROS Pisifera oil palm variety exhibited the lowest tolerance towards Al-toxicity compared to the other oil palm varieties tested.