Journal of Oil Palm Research Vol. Special Issue  2008 July p.  

Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm

Author(s): ABDUL MASANI Mat Yunus* ; HO Chai-Ling** ; AHMAD PARVEEZ Ghulam Kadir*

The construction of transformation vectors carrying bioplastic biosynthetic genes driven by constitutive and oil palm mesocarp-specific promoters was completed. Four planned transformation vectors were produced. The poly-3-hydroxybutyrate (PHB) producing constructs carried the phbA, phbB and phbC genes, while the polyhyroxybutyrate-co-valerate (PHBV) producing constructs carried the bktB, phbB, phbC and tdcB genes. Each of these genes was fused with the transit peptide (Tp) of the oil palm acyl-carrier-protein (ACP) for targeting into the plastids of plant cells. All vectors carry the phosphinothricin acetyltransferase gene (bar) driven by an ubiquitin promoter as plant selectable marker. The matrix attachment region from tobacco (RB7MAR) was also included for stabilization of the transgene expression and to minimize the gene silencing due to positional effects. All constructs were verified by restriction analysis, polymerase chain reaction (PCR) and DNA sequencing.

Keywords: , , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.

** Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering

Cited By

1. Ahmad Parveez, G. K., et al. "Improving Value of Oil Palm using Genetic Engineering." Biocatalysis and Biomolecular Engineering., 2010. 67-81.
2. Bahariah, B., et al. "Biolistic Transformation of Oil Palm using the Phosphomannose Isomerase (Pmi) Gene as a Positive Selectable Marker." Biocatalysis and Agricultural Biotechnology 2(4) (2013): 295-304.
3. Hausmann, T., and I. Broer. "Renewable Polymers in Transgenic Crop Plants." Renewable Polymers: Synthesis, Processing, and Technology., 2011. 247-303.
4. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science 6.September (2015)
5. Masani, M. Y. A., et al. "Regeneration of Viable Oil Palm Plants from Protoplasts by Optimizing Media Components, Growth Regulators and Cultivation Procedures." Plant Science 210 (2013): 118-27.
6. Masani, M. Y. A., et al. "Efficient Transformation of Oil Palm Protoplasts by PEG-Mediated Transfection and DNA Microinjection." PLoS ONE 9(5) (2014)
7. Masani, M. Y. A., et al. "Construction of PHB and PHBV Multiple-Gene Vectors Driven by an Oil Palm Leaf-Specific Promoter." Plasmid 62(3) (2009): 191-200. 8. Mat Yunus, A. M., G. K. Ahmad Parveez, and C. -L Ho. "Review: Transgenic Plants Producing Polyhydroxyalkanoates." Asia-Pacific Journal of Molecular Biology and Biotechnology 16(1) (2008): 1-10.
9. Omidvar, V., et al. "The Oil Palm Metallothionein Promoter Contains a Novel AGTTAGG Motif Conferring its Fruit-Specific Expression and is Inducible by Abiotic Factors." Planta 232(4) (2010): 925-936.
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11. Parveez, G. K. A., et al. "Tissue Culture and Genetic Engineering of Oil Palm." Palm Oil: Production, Processing, Characterization, and Uses., 2012. 87-135.
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13. Teen, Y. J., et al. "Activity Studies, Gene Characterization and Manipulation of 3-Ketothiolase of Oil Palm (Elaeis Guineensis Jacq.) Mesocarp." Journal of Oil Palm Research.SPEC. ISS. JULY (2008): 118-133.
14. Hanin, A. N., M. Y. A. Masani, and G. K. A. Parveez. "Evaluation of Oil Palm Leaf-Specific Promoter (LSP1) Activity for Expressing Phb Genes in Arabidopsis Thaliana." Journal of Oil Palm Research 28(1) (2016): 1-9.

Source: Scopus
Last checked: 22 May 2017

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