Journal of Oil Palm Research Vol. Special Issue  2008 July p.  
DOI:

Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm

Author(s): ABDUL MASANI Mat Yunus* ; HO Chai-Ling** ; AHMAD PARVEEZ Ghulam Kadir*

The construction of transformation vectors carrying bioplastic biosynthetic genes driven by constitutive and oil palm mesocarp-specific promoters was completed. Four planned transformation vectors were produced. The poly-3-hydroxybutyrate (PHB) producing constructs carried the phbA, phbB and phbC genes, while the polyhyroxybutyrate-co-valerate (PHBV) producing constructs carried the bktB, phbB, phbC and tdcB genes. Each of these genes was fused with the transit peptide (Tp) of the oil palm acyl-carrier-protein (ACP) for targeting into the plastids of plant cells. All vectors carry the phosphinothricin acetyltransferase gene (bar) driven by an ubiquitin promoter as plant selectable marker. The matrix attachment region from tobacco (RB7MAR) was also included for stabilization of the transgene expression and to minimize the gene silencing due to positional effects. All constructs were verified by restriction analysis, polymerase chain reaction (PCR) and DNA sequencing.

Keywords: , , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
E-mail: masani@mpob.gov.my

** Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering


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4) Izawati, A.M.D., Masani, M.Y.A., Ismanizan, I., Parveez, G.K.A. Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens (2015) Frontiers in Plant Science, 6 (September), art. no. 727, 10 p.
5) Parveez, G.K.A., Bahariah, B., Ayub, N.H., Masani, M.Y.A., Rasid, O.A., Tarmizi, A.H., Ishak, Z. Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli (2015) Frontiers in Plant Science, 6 (AUG), art. no. 598, 12 p.
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11) Parveez, G.K.A., Rasid, O.A., Hashim, A.T., Ishak, Z., Rosli, S.K., Sambanthamurthi, R. Tissue Culture and Genetic Engineering of Oil Palm (2012) Palm Oil: Production, Processing, Characterization, and Uses, pp. 87-135.
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13) Hausmann, T., Broer, I. Renewable Polymers in Transgenic Crop Plants (2011) Renewable Polymers: Synthesis, Processing, and Technology, pp. 247-303.
14) Ahmad Parveez, G.K., Othman, A., Ramli, U.S., Sambanthamurthi, R., Yunus, A.M.M., Hashim, A.T., Din, A.K., Wahid, M.B. Improving Value of Oil Palm Using Genetic Engineering (2010) Biocatalysis and Biomolecular Engineering, pp. 67-81.
15) Omidvar, V., Abdullah, S.N.A., Izadfard, A., Ho, C.L., Mahmood, M. The oil palm metallothionein promoter contains a novel AGTTAGG motif conferring its fruit-specific expression and is inducible by abiotic factors (2010) Planta, 232 (4), pp. 925-936.
16) Masani, M.Y.A., Parveez, G.K.A., Izawati, A.M.D., Lan, C.P., Siti Nor Akmar, A. Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter (2009) Plasmid, 62 (3), pp. 191-200.
17) Teen, Y.J., Yunus, A.M.M., Parveez, G.K.A., Sambanthamurthi, R. Activity studies, gene characterization and manipulation of 3-ketothiolase of oil palm (elaeis guineensis Jacq.) mesocarp (2008) Journal of Oil Palm Research, (SPEC. ISS. JULY), pp. 118-133.
18) Mat Yunus, A.M., Ahmad Parveez, G.K., Ho, C.-L. Review: Transgenic plants producing polyhydroxyalkanoates (2008) Asia-Pacific Journal of Molecular Biology and Biotechnology, 16 (1), pp. 1-10.

Source: Scopus
Last checked: 30 July 2018

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