Journal of Oil Palm Research Vol. Special Issue  2008 July p.  
Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm

Author(s): ABDUL MASANI Mat Yunus* ; HO Chai-Ling** ; AHMAD PARVEEZ Ghulam Kadir*

The con­struc­tion of trans­for­ma­tion vec­tors car­ry­ing bio­plas­tic biosyn­thetic genes dri­ven by con­sti­tu­tive and oil palm mesocarp-specific pro­mot­ers was com­pleted. Four planned trans­for­ma­tion vec­tors were pro­duced. The poly-3-hydroxybutyrate (PHB) pro­duc­ing con­structs car­ried the phbA, phbB and phbC genes, while the polyhyroxybutyrate-co-valerate (PHBV) pro­duc­ing con­structs car­ried the bktB, phbB, phbC and tdcB genes. Each of these genes was fused with the tran­sit pep­tide (Tp) of the oil palm acyl-carrier-protein (ACP) for tar­get­ing into the plas­tids of plant cells. All vec­tors carry the phos­phinothricin acetyl­trans­ferase gene (bar) dri­ven by an ubiq­ui­tin pro­moter as plant selec­table marker. The matrix attach­ment region from tobacco (RB7MAR) was also included for sta­bi­liza­tion of the trans­gene expres­sion and to min­i­mize the gene silenc­ing due to posi­tional effects. All con­structs were ver­i­fied by restric­tion analy­sis, poly­merase chain reac­tion (PCR) and DNA sequencing.

Keywords: , , , , ,

Author Information
* Malaysian Palm Oil Board, P. O. Box 10620, 50720 Kuala Lumpur, Malaysia.
E-mail: masani@mpob.gov.my

** Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering


Cited By

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2. Bahariah, B., et al. "Biolistic Transformation of Oil Palm using the Phosphomannose Isomerase (Pmi) Gene as a Positive Selectable Marker." Biocatalysis and Agricultural Biotechnology 2(4) (2013): 295-304.
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4. Izawati, A. M. D., et al. "Evaluation on the Effectiveness of 2-Deoxyglucose-6-Phosphate Phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis Guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium Tumefaciens." Frontiers in Plant Science 6.September (2015)
5. Masani, M. Y. A., et al. "Regeneration of Viable Oil Palm Plants from Protoplasts by Optimizing Media Components, Growth Regulators and Cultivation Procedures." Plant Science 210 (2013): 118-27.
6. Masani, M. Y. A., et al. "Efficient Transformation of Oil Palm Protoplasts by PEG-Mediated Transfection and DNA Microinjection." PLoS ONE 9(5) (2014)
7. Masani, M. Y. A., et al. "Construction of PHB and PHBV Multiple-Gene Vectors Driven by an Oil Palm Leaf-Specific Promoter." Plasmid 62(3) (2009): 191-200. 8. Mat Yunus, A. M., G. K. Ahmad Parveez, and C. -L Ho. "Review: Transgenic Plants Producing Polyhydroxyalkanoates." Asia-Pacific Journal of Molecular Biology and Biotechnology 16(1) (2008): 1-10.
9. Omidvar, V., et al. "The Oil Palm Metallothionein Promoter Contains a Novel AGTTAGG Motif Conferring its Fruit-Specific Expression and is Inducible by Abiotic Factors." Planta 232(4) (2010): 925-936.
10. Parveez, G. K. A., et al. "Production of Polyhydroxybutyrate in Oil Palm (Elaeis Guineensis Jacq.) Mediated by Microprojectile Bombardment of PHB Biosynthesis Genes into Embryogenic Calli." Frontiers in Plant Science 6(AUG) (2015)
11. Parveez, G. K. A., et al. "Tissue Culture and Genetic Engineering of Oil Palm." Palm Oil: Production, Processing, Characterization, and Uses., 2012. 87-135.
12. Parveez, G. K. A., et al. "Biotechnology of Oil Palm: Strategies Towards Manipulation of Lipid Content and Composition." Plant Cell Reports 34(4) (2015): 533-543.
13. Teen, Y. J., et al. "Activity Studies, Gene Characterization and Manipulation of 3-Ketothiolase of Oil Palm (Elaeis Guineensis Jacq.) Mesocarp." Journal of Oil Palm Research.SPEC. ISS. JULY (2008): 118-133.
14. Hanin, A. N., M. Y. A. Masani, and G. K. A. Parveez. "Evaluation of Oil Palm Leaf-Specific Promoter (LSP1) Activity for Expressing Phb Genes in Arabidopsis Thaliana." Journal of Oil Palm Research 28(1) (2016): 1-9.

Source: Scopus
Last updated: 27 June 2016

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