Journal of Oil Palm Research Vol. 29  March 2017 p.  23-34
DOI:

DIFFERENTIAL EXPRESSION ANALYSIS OF OIL PALM FATTY ACID BIOSYNTHETIC ENZYMES WITH GEL-FREE QUANTITATIVE PROTEOMICS

Author(s): BENJAMIN YII CHUNG, LAU*; JAMES D MORTON**; SANTANU DEB-CHOUDHURYǂ; STEFAN CLERENSǂ; JOLON M DYERǂ and UMI SALAMAH RAMLI*

The con­trol mech­a­nisms of de novo fatty acid biosyn­the­sis con­tinue to intrigue, par­tic­u­larly at pro­tein level. The cross talk between tran­scrip­tional and meta­bolic reg­u­la­tory net­works remains unde­fined. In order to under­stand the meta­bolic fac­tors affect­ing oleic acid biosyn­the­sis in Elaeis guineen­sis var. ten­era (oil palm), two-dimensional liq­uid chromatography-tandem mass spec­trom­e­try (2DLC-MS/MS) was attempted to cap­ture the main fatty acid biosyn­thetic enzymes involved in oleic acid pro­duc­tion. These enzymes were extracted from the chro­mo­plast of low and high oleic meso­carps of 20th week after anthe­sis. Chro­mo­plast pro­teins were sep­a­rated using offline 2DLC for mass spec­tro­met­ric analy­sis. Two sub­units of acetyl-CoA car­boxy­lase, β-ketoacyl-acyl car­rier pro­tein (ACP) reduc­tase, β-hydroxyacyl-ACP dehy­dro­ge­nase, 3-enoyl– ACP reduc­tase, β-ketoacyl-ACP syn­thase and stearoyl-ACP desat­urase were iden­ti­fied. The rel­a­tive expres­sion of these fatty acid biosyn­thetic enzymes was deter­mined using iso­baric tags labelling. Ini­tial assess­ment revealed that β-hydroxyacyl-ACP dehy­dro­ge­nase and β-ketoacyl-ACP syn­thase were present in a higher abun­dance in the high oleic acid meso­carps while the accu­mu­la­tion of 3-enoyl-ACP reduc­tase was lower rel­a­tive to the other fatty acid biosyn­thetic enzymes inves­ti­gated. The exis­tence of other dif­fer­en­tially expressed meta­bolic enzymes sug­gests that the con­trol mech­a­nism of fatty acid pro­duc­tion, in par­tic­u­lar oleic acid, might involve more than just the main fatty acid biosyn­thetic enzymes.

Keywords: , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail:


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