Journal of Oil Palm Research Vol. 29  March 2017 p.  81-87
DOI:

ENZYMATIC SYNTHESIS OF STRUCTURED LIPID BASED ON SILKWORM OIL AND PALM OLEIN

Author(s): THUMU RAVINDER*; SHIVA SHANKER KAKI*; PRABHAKAR, I N S S*; RAO, B V S K *; SARAT KUMAR SWAIN** and PRASAD, R B N*

The present study describes the syn­the­sis and char­ac­ter­i­sa­tion of struc­tured lipid (SL) con­tain­ing α-linolenic acid (ALA) pre­pared from palm olein (POo) and silk­worm oils (SWO) employ­ing enzy­matic inter­ester­i­fi­ca­tion method. POo was cho­sen as one of the sub­strate because it does not con­tain ALA and is oxida­tively more sta­ble com­pared to other veg­etable oils. The study was aimed to obtain an ALA con­tent of about 10% in the struc­tured lipid which was achieved with the blend of POo and SWO in the ratio of 80:20 (wt/wt). The reac­tions were catal­ysed by a sn-1, 3-regiospecific lipase, Lipozyme RM IM. The effect of reac­tion time, tem­per­a­ture were stud­ied and were found to be at 4 hr and 65°C-70°C respec­tively at a lipase con­cen­tra­tion 4% (w/w) of sub­strates. Physico-chemical char­ac­ter­is­tics of both the sub­strate oils were deter­mined and com­pared with those of ini­tial phys­i­cal blends and the pre­pared SL. The oxida­tive sta­bil­ity of SL was also stud­ied and the induc­tion time was found to be at 13.2 hr which was higher with respect to SWO. The SL pre­pared using refined POo, SWO has poten­tial appli­ca­tions in nutraceu­ti­cal, food and indus­trial applications.

Keywords: , , , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail: zuliana@mpob.gov.my


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