Journal of Oil Palm Research Vol. 28  March 2016 p.  1-9
DOI: https://doi.org/10.21894/jopr.2016.2801.01

EVALUATION OF OIL PALM LEAF-SPECIFIC PROMOTER (LSP1) ACTIVITY FOR EXPRESSING PHB GENES IN Arabidopsis thaliana

Author(s): AYUB NOR HANIN*; MAT YUNUS ABDUL MASANI* and GHULAM KADIR AHMAD PARVEEZ*

Pro­duc­tion of poly­hy­drox­y­bu­tyrate (PHB), a biodegrad­able plas­tic, in plant, has been stud­ied all over the world. We have con­structed a trans­for­ma­tion vec­tor pLSP15 car­ry­ing the PHB biosyn­the­sis genes. Each of PHB genes was dri­ven by an oil palm leaf-specific pro­moter (LSP1). This trans­for­ma­tion vec­tor was later trans­formed into Ara­bidop­sis thaliana and suc­cess­fully pro­duced T3 puta­tive trans­genic plants. The mature T3 gen­er­a­tion was analysed by Reverse Transcription-qPCR (RT-qPCR) to test the capa­bil­ity of a mono­cotyle­don oil palm LSP1 pro­moter in dri­ving the expres­sion of the PHB genes in a dicotyle­don A. thaliana plant. Expres­sion of all PHB genes, i.e. phbA (2.32), phbB (1.53) and phbC (1.47) rel­a­tive to the wild-type, were detected in the plant. Nile blue A stain­ing demon­strated that the PHB poly­mer was suc­cess­fully pro­duced through­out the devel­op­men­tal stage of the trans­genic A. thaliana plant with­out any dele­te­ri­ous effects. The results demon­strated that the oil palm LSP1 pro­moter could drive the expres­sion of the PHB biosyn­the­sis genes in plants.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang,Selangor, Malaysia. E-mail: masani@mpob.gov.my


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