Journal of Oil Palm Research Vol. 28  March 2016 p.  1-9
DOI: https://doi.org/10.21894/jopr.2016.2801.01

EVALUATION OF OIL PALM LEAF-SPECIFIC PROMOTER (LSP1) ACTIVITY FOR EXPRESSING PHB GENES IN Arabidopsis thaliana

Author(s): AYUB NOR HANIN*; MAT YUNUS ABDUL MASANI* and GHULAM KADIR AHMAD PARVEEZ*

Production of polyhydroxybutyrate (PHB), a biodegradable plastic, in plant, has been studied all over the world. We have constructed a transformation vector pLSP15 carrying the PHB biosynthesis genes. Each of PHB genes was driven by an oil palm leaf-specific promoter (LSP1). This transformation vector was later transformed into Arabidopsis thaliana and successfully produced T3 putative transgenic plants. The mature T3 generation was analysed by Reverse Transcription-qPCR (RT-qPCR) to test the capability of a monocotyledon oil palm LSP1 promoter in driving the expression of the PHB genes in a dicotyledon A. thaliana plant. Expression of all PHB genes, i.e. phbA (2.32), phbB (1.53) and phbC (1.47) relative to the wild-type, were detected in the plant. Nile blue A staining demonstrated that the PHB polymer was successfully produced throughout the developmental stage of the transgenic A. thaliana plant without any deleterious effects. The results demonstrated that the oil palm LSP1 promoter could drive the expression of the PHB biosynthesis genes in plants.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang,Selangor, Malaysia. E-mail: masani@mpob.gov.my


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