Journal of Oil Palm Research Vol. 29  March 2017 p.  35-46
DOI:

GENETIC VARIATION OF ENTOMOPATHOGENIC FUNGI, Metarhizium anisopliae AND Isaria amoenerosea AND THEIR PATHOGENICITY AGAINST SUBTERRANEAN TERMITE, Coptotermes curvignathus

Author(s): PONG KUAN KIN*; RAMLE MOSLIM**; WAHIZATUL AFZAN AZMI*; NORMAN KAMARUDIN** and SITI RAMLAH AHMAD ALI**

This study deter­mined the genetic sim­i­lar­ity of 22 iso­lates of Metarhiz­ium aniso­pliae and 51 iso­lates of Isaria amoen­erosea iso­lated from min­eral and peat soils, involv­ing ran­dom ampli­fied poly­mor­phic DNA (RAPD) and using poly­merase chain reac­tion (PCR) tech­nol­ogy. The path­o­genic­ity of the selected iso­lates from both gen­era of fungi were then tested against the sub­ter­ranean ter­mites, Cop­toter­mes curvi­g­nathus. The RAPD-PCR analy­sis revealed high genetic sim­i­lar­ity within the iso­lates of M. aniso­pliae and I. amoen­erosea, at the sim­i­lar­ity rang­ing from 52% to 100%. For M. aniso­pliae, the fun­gal iso­lates were grouped into two main clus­ters and the iso­lates of I. amoen­erosea were sep­a­rated into five main clus­ters. Within clus­ters in each species, there was lit­tle evi­dence on high sim­i­lar­ity within iso­lates from the same soil type, but most of fun­gal iso­lates were well dis­trib­uted within the clus­ters. Based on the bioas­say data, the iso­lates of M. aniso­pliae were more path­o­genic on ter­mites, as com­pared to the iso­lates of I. amoen­erosea. The iso­lates of M. aniso­pliae caused high per­cent­age of infec­tions, rang­ing from 71% to 84% at 15 days after treat­ment (DAT), and the iso­late of M11H was the most path­o­genic against ter­mites with the LT50 value of 6.36 days. For iso­lates of I. amoen­erosea, the per­cent­age of infec­tion were low, rang­ing from only 5% to 31%. This lab­o­ra­tory study showed that M. aniso­pliae has a poten­tial to be used to con­trol ter­mites, as it has rapid infect­ing capa­bil­ity. How­ever, fur­ther stud­ies should be con­ducted to con­firm the effec­tive­ness of the M. aniso­pliae to con­trol ter­mites, espe­cially in the field.

Keywords: , , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia. E-mail:


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