This study evaluated the effects of organic matter techniques application (surface pile and subsurface biopore infiltration holes) and organic matter types on the vertical root distribution of oil palm (Elaeis guineensis). The experiment used a factorial randomised block design with two factors: Application technique (pile and biopore infiltration holes) and five organic matter types (no organic matter, fresh empty fruit bunches [EFB], EFB compost, palm fronds and palm leaves). Root depth was measured at the second and fourth months after treatment. Although statistical analysis showed no significant differences (p>0.05), consistent biological trends were observed. Treatments using EFB compost, particularly with the pile method, indicated a tendency to promote root growth closer to the soil surface, with root depth decreasing from 6.33 to 4.17 cm. Pile applications showed faster response of root distribution than biopore infiltration holes, although biopore infiltration holes combined with EFB compost also improved root distribution. Conversely, treatments without organic inputs or with slowly decomposing materials (fresh EFB, palm fronds and palm leaves) resulted in minimal changes in root depth. These findings suggest that using EFB compost with appropriate application techniques may enhance shallow root proliferation, potentially improve nutrient uptake efficiency and support sustainable plantation management.