Journal of Oil Palm Research Vol.   p.  
DOI: https://doi.org/10.21894/jopr.2020.0067

EXTRACTION AND PURIFICATION OF PHYTOSTEROLS MIXTURE FROM PALM FATTY ACID DISTILLATE (PFAD) USING MULTISTAGE EXTRACTION PROCESSES

Author(s): NOR FAIZAH JALANI*; NOORSHAMSIANA ABDUL WAHAB*; WAN HASAMUDIN WAN HASSAN*; KAMARUDIN HASSAN*; NAHRUL HAYAWIN ZAINAL* and MOHAMAD FAIZAL IBRAHIM**

Phytosterols are among the bioactive compounds naturally present in vegetable oils and their by-products or derivatives. A phytosterol resource (PSR), solid by-product from the extraction of vitamin E in palm fatty acid distillate (PFAD), contains 2%-4% (w/w) total sterols. Therefore, the extraction of phytosterols from the PSR in a mini-pilot scale involving multistage extraction processes was developed to recover the valuable minor component. The multistage extraction and purification processes comprised of solid-liquid extraction (SLE) with hexane at 35°C in 1 hr, saponification reaction at the reflux temperature of 80°C for 1 hr, liquidliquid extraction (LLE) with hexane and water, and crystallisation and vacuum filtration at -5°C for 20 hr. On average, gas chromatographic (GC) analysis showed the phytosterols recovered from the extraction and purification process had more than 80% purity. The recovery of total sterols from the PSR was 84% composed of β-sitosterol (21%-22%), campesterol (13%-20%) and stigmasterol (59%-64%). This extraction process is technically feasible to extract and produce crude phytosterols from a PFAD by-product.

Keywords: , , ,

Author Information
* Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
E-mail: norfaizah@mpob.gov.my

** Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.


Cited By

Call For Papers

Article In Press

Search for:


Most cited articles


SIGN UP To JOPR MAILING LIST

Subscribe with us to get the latest information on Palm Oil Research from MPOB today!