ARTICLE IN PRESS

BACTERIAL CELLULOSE PRODUCTION FROM OIL PALM FROND JUICE AND ITS IMPREGNATION WITH SILVER NANOPARTICLES FOR ANTIBACTERIAL WOUND DRESSING

TAN SOU MIN1; NOR SYAHIRAH ABD KARIM1; SHAHRIL MOHAMAD1; JUNAIDI ZAKARIA1; MOHD HAFIZ ARZMI2; HASSAN FAHMI ISMAIL3 and ROHANA ABU1*

DOI: https://doi.org/10.21894/jopr.2023.0055
Received: 7 April 2023   Accepted: 20 August 2023   Published Online: 26 October 2023
ABSTRACT

Bacterial cellulose-silver nanoparticles (BC-AgNPs) composite was prepared for a bacterial wound dressing. The oil palm frond (OPF) juice was utilised as a low-cost raw material for BC production. The AgNPs were incorporated in the BC composite by thermal reduction of 1 mM silver nitrate for their antibacterial agent. The BC-AgNPs composite had dense nanofibrils with an average diameter of 61.5 ± 1.0 nm as shown in the field emission scanning electron microscope (FESEM) images. The composite had a crystallinity index of 86.5% and the nanoparticles had a face-centred cubic geometry with a crystal size of 26.5 nm as determined by X-ray diffraction analysis (XRD). The Ag content was 1.463 mg/100 cm2 in the composite analysed by atomic absorption spectrophotometry (AAS). 10.4% of the total Ag content was released from AgNPs in 72 hr as measured by inductively coupled plasma mass spectrometry (ICP-MS). The composite also demonstrated excellent antibacterial action against Staphylococcus aureus, giving a 29 ± 0.8 mm inhibition zone by the disk diffusion assay. Pure BC composite exhibited no cytotoxicity effect on the HSF1184 fibroblast cells and 10%-40% BC-AgNPs composite extracts were compatible with the cell growth. The study suggests the BCAgNPs composite is a good material for antibacterial wound dressing.

KEYWORDS:


1 Faculty of Chemical and Process Engineering Technology,
Universiti Malaysia Pahang Al-Sultan Abdullah,
26300 Kuantan, Pahang, Malaysia.

2 Department of Fundamental Dental and Medical Sciences,
Kulliyyah of Dentistry, International Islamic University
Malaysia, 25200 Kuantan, Pahang, Malaysia.

3 Institute of Marine Biotechnology, Universiti Malaysia
Terengganu, 21030 Kuala Terengganu,
Terengganu, Malaysia.

* Corresponding author e-mail: rohanaa@umpsa.edu.my