Oil palm is an oil producing tropical plant. While it is the highest yielding oil producing plant, it takes three years to yield. Therefore there is an urgent need for controlled production for oil palm suspension cells in a bioreactor to support the clonal propagation of elite planting materials. The propagation of the cells is affected by various culture conditions. In this study, we examined the effect of two operating parameters – agitation rate (80 to 335 rpm) and aeration (10% to 80% dissolved oxygen) – on the proliferation of suspension cells. The sugar consumption, production of biomass, amino acids and organic acids were monitored. At 43% dissolved oxygen, two of the agitation rates (120 rpm and 225 rpm) resulted in more than 200% increase in biomass compared to the initial inoculum. This study indicates that the bioreactor operating parameters between 120-300 rpm and 20%-80% dissolved oxygen are acceptable for the culture of oil palm cells.