RESEARCH ARTICLE

Journal of Oil Palm Research Vol. 8 No. 1, December 1996, p. 29-36

FLUORESCENCE in situ HYBRIDIZATION OF RRNA PROBE TO Elaeis guineensis (Tenera) CHROMOSOMES

MARIA Madon*; CLYDE, M M+; CHEAH, S C*

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ABSTRACT

Fluorescence in situ hybridization (FISH) was performed on Elaeis guineensis (tenera) chromosomes using the highly repetitive ribosomal ribonucleic acid (rRNA) gene probe, pBG 35, from flax. FISH of pBG 35 on interphase cells showed two hybridization sites, thereby indicating two nucleoli, while FISH on metaphase chromosome spread showed the presence of four nucleolar organizer regions (NORs).

KEYWORDS: , , ,


* Palm Oil Research Institute of Malaysia,
P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.

** Genetics Department, Faculty of Life Sciences,
Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

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