RESEARCH ARTICLE

Journal of Oil Palm Research Vol. 8 No. 1, December 1996, p. Oct-19

HISTOPATHOLOGY OF Metisa plana (LEPIDOPTERA: PSYCHIDAE) INFECTED WITH Beauveria bassiana DEUTEROMYCOTINA: HYPHOMYCETES)

RAMLE Moslim*; SITI RAMLAH Ahmad Ali*; MOHD BASRI Wahid*

ABSTRACT

Fourth larval instars of Metisa plana Walker were sprayed with conidial suspensions of Beauveria bassiana(Balsamo) Vuillemin containing 6.25 x 10-6 conidial/ml. The mode of fungal infection was determined histologically. At 48 hours after inoculation, fungal hyphae penetrated the integument inside the trachea and via the nuclei of epidermal cells. Fungal hyphae infiltrated the fat bodies underlying the integument. At 72 hours after inoculation, the fat tissues were damaged by progressive colonization with hyphae. Subsequently, the hyphae invaded the muscle tissues, neural tissues, germ cavities, Malpighian tubules, gut musculature and epithelial cells, and finally colonized the gut lumen. Between 96 and 120 hours post inoculation, all internal organs of the bagworm were heavily colonized with hyphae, and the infected bagworms were already dead. Twenty-four hours after death, whitish mycelia began to emerge from the cuticle of the bagworm cadavers.

KEYWORDS:


* Palm Oil Research Institute of Malaysia,
P.O. Box 10620, 50720 Kuala Lumpur, Malaysia.

Releated

REFINING OF RED PALM OIL

A process has been developed for the refining of crude palm oil (CPO) to produce Red Palm Oil (RPO) without destroying the carotenes. It involves a pretreatment of CPO followed by deacidification and deodorization using molecular distillation. The RPO produced has less than 0.1% of free fatty acid (FFA) and retains more than 80% of […]

FLUORESCENCE in situ HYBRIDIZATION OF RRNA PROBE TO Elaeis guineensis (Tenera) CHROMOSOMES

Fluorescence in situ hybridization (FISH) was performed on Elaeis guineensis (tenera) chromosomes using the highly repetitive ribosomal ribonucleic acid (rRNA) gene probe, pBG 35, from flax. FISH of pBG 35 on interphase cells showed two hybridization sites, thereby indicating two nucleoli, while FISH on metaphase chromosome spread showed the presence of four nucleolar organizer regions […]