RESEARCH ARTICLE

Journal of Oil Palm Research (Special Issue - July 2008), p. 77-86

TRANSFORMATION OF PHB AND PHBV GENES DRIVEN BY MAIZE UBIQUITIN PROMOTER INTO OIL PALM FOR THE PRODUCTION OF BIODEGRADABLE PLASTICS

AHMAD PARVEEZ Ghulam Kadir* ; BAHARIAH Bohari* ; NOR HANIN Ayub* ; ABDUL MASANI Mat Yunus* ; OMAR Abdul Rasid* ; AHMAD TARMIZI Hashim* ; ZAMZURI Ishak* ; MOHAMAD ARIF Abd Manaf* ; AHMAD KUSHAIRI Din* ; YORK, Gregory**; YEONG Bae Jo**; SINSKEY, Anthony J.**

ABSTRACT

Three bacterial genes coding for the enzymes, 3-ketothiolase (bktB), acetoacetyl-CoA reductase (phaB) and PHB synthase (phaC), required for the synthesis of PHB from acetyl-CoA in bacteria were transformed into oil palm embryogenic calli. For the production of copolymer polyhydroxybutyrate–co-valerate (PHBV), the threonine dehydratase (tdcB) gene from Escherichia coli was also transformed into oil palm embryogenic calli for producing propionyl-CoA, the substrate for hydroxyvalerate. These genes were under the control of the maize ubiquitin promoter. Currently, many transformed embryogenic lines resistant to the herbicide Basta have already been produced. These transformed calli were later regenerated to produce a few hundred plantlets which are now growing in a biosafety screenhouse. Molecular analyses have demonstrated stable integration of the transgenes in their genome.

KEYWORDS:


* Malaysian Palm Oil Board,
P. O. Box 10620,
50720 Kuala Lumpur,
Malaysia.
E-mail: parveez@mpob.gov.my

** Department of Biology,
Massachusetts Institute of Technology,
77 Massachusetts Avenue,
Cambridge, MA 02139,
USA.

Journal of Oil Palm Research Special Issue on Malaysia-MIT Biotechnology Partnership Programme: Volume 2 - Oil Palm Metabolic Engineering

Releated

CAROTENOID PROFILES AND PRELIMINARY INVESTIGATION ON CAROTENOID BIOSYNTHESIS IN THE OIL PALM (Elaeis guineensis) MESOCARP

The changes in carotenoid content and profile were studied in oil palm (E. guineensis) mesocarp at various stages of development. Spectrophotometric analysis showed that chlorophyll synthesis predominates in the young fruits but it then shifts strongly to carotenoids as the fruit ripens. The major carotenoids in the oil from ripe fruits are α- and β-carotenes. […]

MESOCARP-SPECIFIC METALLOTHIONEIN-LIKE GENE PROMOTER FOR GENETIC ENGINEERING OF OIL PALM

Primers from within the coding region were used to capture the 5′ regulatory sequence of the mesocarpspecific metallothionein-like gene, MT3-A, via PCR-based genome walking. The amplified 1040 bp genomic fragment was cloned and sequenced. The sequence of the genomic clone showed total homology with the MT3-A cDNA sequence within their overlapping regions. Rapid amplification of […]